Filogenia e taxonomia de mixozoários parasitos de peixes provenientes de rios interiores do Estado de São Paulo
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Data
2020-02-18
Autores
Vieira, Diego Henrique Mirandola Dias [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
Parasitos de organismos aquáticos apresentam considerável relevância para estudo devido ao seu impacto nos sistemas de aquicultura e na pesca em todo o mundo. Mixozoários são microparasitos que tem um ciclo de vida complexo, alternando entre um hospedeiro vertebrado e um invertebrado, podendo ser responsáveis por causar doenças principalmente em peixes, com altas taxas de mortalidade. Muitos mixozoários permanecem desconhecidos pela comunidade científica e seu potencial em causar doenças em peixes segue indeterminado em alguns casos. Desta forma, o presente trabalho teve como objetivo analisar de forma morfológica e molecular mixozoários parasitos de peixes de rios interiores do estado de São Paulo, assim como caracterizar as relações filogenéticas desses parasitos e seu potencial patológico. Durante os anos de 2016 a 2019 foram coletados espécimes de cinco espécies de peixes das ordens Characiformes e Siluriformes. Foram utilizadas técnicas de biologia molecular, histologia e análise ultraestrutural na descrição taxonômica das espécies. Myxobolus batalhensis n. sp. encontrado parasitando Salminus hilarii (Bryconidae), Myxobolus imparfinis n. sp. encontrando parasitando Imparfinis mirini (Heptapteridae), Henneguya sp. 1 encontrado parasitando Prochilodus lineatus (Prochilodontidae) e Henneguya sp. 2 encontrado parasitando Astyanax lacustris (Characidae), tiveram a caracterização morfológica e molecular realizadas. Foi feito um estudo suplementar sobre Henneguya guanduensis, encontrado parasitando Hoplosternum littorale (Callichthyidae), com análise molecular, ultraestrutural e filogenética. Foi realizada ainda a análise filogenética das espécies de mixozoários da família Myxobolidae que parasitam peixes do Brasil. A diversidade de mixozoários encontrados nas espécies de peixes alvos deste estudo evidencia a necessidade de se intensificar o estudo da diversidade e do potencial patogênico destes parasitos.
Parasites of aquatic organisms present considerable relevance to study because of their impact on aquaculture and fisheries worldwide. Myxozoans are microparasites that have a complex life cycle, alternating between a vertebrate and an invertebrate host, being able to be responsible for causing diseases mainly in fish, with high mortality rates. Many myxozoans remain unknown to the scientific community and their potential to cause disease in fish remains undetermined in some cases. Thus, the present study aimed to analyze morphologically and molecularly myxozoans parasites of fish from inland rivers of the state of São Paulo, as well as to characterize the phylogenetic relationships of these parasites and their pathological potential. From 2016 to 2019 specimens from five species from Characiformes and Siluriformes orders were collected. Molecular biology, histology, and ultrastructural analysis techniques were used taxonomic description of species. Myxobolus batalhensis n. sp. found parasitizing Salminus hilarii (Bryconidae), Myxobolus imparfinis n. sp. found parasitizing Imparfinis mirini (Heptapteridae), Henneguya sp. 1 found parasitizing Prochilodus lineatus (Prochilodontidae) and Henneguya sp. 2 found parasitizing Astyanax lacustris (Characidae), had the morphological and molecular characterization performed. A supplementary study on Henneguya guanduensis found parasitizing Hoplosternum littorale (Callichthyidae) was done, with molecular, ultrastructural and phylogenetic analysis. A phylogenetic analysis of the myxozoans species of Myxobolidae family parasitizing fish from Brazil was performed. The diversity of myxozoans found in the target fish species of this study highlights the need to intensify the study of the diversity and pathogenic potential of these parasites.
Parasites of aquatic organisms present considerable relevance to study because of their impact on aquaculture and fisheries worldwide. Myxozoans are microparasites that have a complex life cycle, alternating between a vertebrate and an invertebrate host, being able to be responsible for causing diseases mainly in fish, with high mortality rates. Many myxozoans remain unknown to the scientific community and their potential to cause disease in fish remains undetermined in some cases. Thus, the present study aimed to analyze morphologically and molecularly myxozoans parasites of fish from inland rivers of the state of São Paulo, as well as to characterize the phylogenetic relationships of these parasites and their pathological potential. From 2016 to 2019 specimens from five species from Characiformes and Siluriformes orders were collected. Molecular biology, histology, and ultrastructural analysis techniques were used taxonomic description of species. Myxobolus batalhensis n. sp. found parasitizing Salminus hilarii (Bryconidae), Myxobolus imparfinis n. sp. found parasitizing Imparfinis mirini (Heptapteridae), Henneguya sp. 1 found parasitizing Prochilodus lineatus (Prochilodontidae) and Henneguya sp. 2 found parasitizing Astyanax lacustris (Characidae), had the morphological and molecular characterization performed. A supplementary study on Henneguya guanduensis found parasitizing Hoplosternum littorale (Callichthyidae) was done, with molecular, ultrastructural and phylogenetic analysis. A phylogenetic analysis of the myxozoans species of Myxobolidae family parasitizing fish from Brazil was performed. The diversity of myxozoans found in the target fish species of this study highlights the need to intensify the study of the diversity and pathogenic potential of these parasites.
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Palavras-chave
Análise ultraestrutural, biologia molecular, histologia, filogenia, Myxobolidae, rio Batalha, rio Tietê, SSU rDNA