Mudanças na ultra-estrutura da parede celular de mangas 'Tommy Atkins' tratadas com cloreto de cálcio na pré-colheita
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Data
2002-04-01
Autores
Evangelista, Regina Marta [UNESP]
CHITARRA, ADIMILSON BOSCO
CHITARRA, MARIA ISABEL FERNANDES
Título da Revista
ISSN da Revista
Título de Volume
Editor
Sociedade Brasileira de Fruticultura
Resumo
Mangas 'Tommy Atkins' produzidas na região de Ibirá, São Paulo, foram pulverizadas na pré-colheita com cloreto de cálcio, nas concentrações de 0,0%, 2,5% e 5,0%, em três épocas de seu desenvolvimento (40; 60 e 90 dias após a floração) a fim de verificar a influência do cálcio na estrutura da parede celular destes frutos através de microscopia eletrônica de transmissão, imediatamente após a colheita e depois de 35 dias de armazenamento. Para fixar o material da polpa, utilizou-se metodologia descrita por Jacob e Gowanlock (1995). Nas condições experimentais, verificou-se que os frutos do tratamento-controle (sem cloreto de cálcio), no dia da colheita, já apresentavam desestruturação da parede celular e dissolução da lamela média (LM). A degradação da parede celular ocorre inicialmente na LM, levando à formação de espaços vazios bastante distintos, apresentando uma dissolução ainda maior, com o armazenamento prolongado (35 dias). Os frutos tratados com cloreto de cálcio a 5,0% apresentaram uma LM bem definida e ausência de espaços vazios, mesmo após o armazenamento, mostrando ser uma concentração efetiva na preservação da lamela média.
Mango 'Tommy Atkins ' produced in Ibirá, São Paulo, was treated at preharvest time with concentrations of 0,0%, 2,5% and 5,0% calcium chloride at three times of its development (40, 60 and 90 days after antesis) in order to identify the influence of calcium on the structure of the cell wall, by using transmission electron microscopy (TEM), immediately after the harvest and at 35 days of storage. The methodology described by Jacob and Gowanlock (1995) was used to fix the pulp material. It was observed that untreated fruits (without calcium chloride) showed desintegration of the cell wall and breakup of the middle lamella (LM) at the harvest time. The degradation of the LM site starts with the formation of quite different empty spaces, whose breakups are larger when prolonging storage for 35 days. The treated fruits with calcium chloride at 5,0% presented a defined LM and absence of empty spaces, even after storage. The application of calcium chloride at 5,0% was effective to preserve the middle lamella structure.
Mango 'Tommy Atkins ' produced in Ibirá, São Paulo, was treated at preharvest time with concentrations of 0,0%, 2,5% and 5,0% calcium chloride at three times of its development (40, 60 and 90 days after antesis) in order to identify the influence of calcium on the structure of the cell wall, by using transmission electron microscopy (TEM), immediately after the harvest and at 35 days of storage. The methodology described by Jacob and Gowanlock (1995) was used to fix the pulp material. It was observed that untreated fruits (without calcium chloride) showed desintegration of the cell wall and breakup of the middle lamella (LM) at the harvest time. The degradation of the LM site starts with the formation of quite different empty spaces, whose breakups are larger when prolonging storage for 35 days. The treated fruits with calcium chloride at 5,0% presented a defined LM and absence of empty spaces, even after storage. The application of calcium chloride at 5,0% was effective to preserve the middle lamella structure.
Descrição
Palavras-chave
manga, amadurecimento, lamela média, armazenamento, mango, ripening, middle lamella, storage
Como citar
Revista Brasileira de Fruticultura. Sociedade Brasileira de Fruticultura, v. 24, n. 1, p. 254-257, 2002.