Spatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebrafish (Danio rerio)
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2008-07-01
Autores
Alves-Costa, Fernanda A. [UNESP]
Denovan-Wright, Eileen M.
Thisse, Christine
Thisse, Bernard
Wright, Jonathan M.
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Resumo
We have determined the structure of the fatty acid-binding protein 6 (fabp6) gene and the tissue-specific distribution of its transcripts in embryos, larvae and adult zebrafish (Danio rerio). Like most members of the vertebrate FABP multigene family, the zebrafish fabp6 gene contains four exons separated by three introns. The coding region of the gene and expressed sequence tags code for a polypeptide of 131 amino acids (14 kDa, pI 6.59). The putative zebrafish Fabp6 protein shared greatest sequence identity with human FABP6 (55.3%) compared to other orthologous mammalian FABPs and paralogous zebrafish Fabps. Phylogenetic analysis showed that the zebrafish Fabp6 formed a distinct clade with the mammalian FABP6s. The zebrafish fabp6 gene was assigned to linkage group (chromosome) 21 by radiation hybrid mapping. Conserved gene synteny was evident between the zebrafish fabp6 gene on chromosome 21 and the FABP6/Fabp6 genes on human chromosome 5, rat chromosome 10 and mouse chromosome 11. Zebrafish fabp6 transcripts were first detected in the distal region of the intestine of embryos at 72 h postfertilization. This spatial distribution remained constant to 7-day-old larvae, the last stage assayed during larval development. In adult zebrafish, fabp6 transcripts were detected by RT-PCR in RNA extracted from liver, heart, intestine, ovary and kidney (most likely adrenal tissue), but not in RNA from skin, brain, gill, eye or muscle. In situ hybridization of a fabp6 riboprobe to adult zebrafish sections revealed intense hybridization signals in the adrenal homolog of the kidney and the distal region of the intestine, and to a lesser extent in ovary and liver, a transcript distribution that is similar, but not identical, to that seen for the mammalian FABP6/Fabp6 gene. © 2008 The Authors.
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Palavras-chave
Adrenal gland, Conserved gene synteny, Ileum, In situ hybridization, Linkage group assignment, fatty acid binding protein, fatty acid binding protein 6, amino acid sequence, chromosome 11, chromosome 21, chromosome 5, controlled study, developmental stage, exon, expressed sequence tag, genetic linkage, genetic transcription, in situ hybridization, nonhuman, nucleotide sequence, phylogeny, priority journal, radiation hybrid mapping, reverse transcription polymerase chain reaction, tissue distribution, zebra fish, Amino Acid Sequence, Animals, Base Sequence, DNA, Complementary, Fatty Acid-Binding Proteins, Gastrointestinal Hormones, Gene Expression Regulation, Developmental, In Situ Hybridization, Molecular Sequence Data, Multigene Family, Phylogeny, Sequence Homology, Amino Acid, Time Factors, Tissue Distribution, Transcription, Genetic, Zebrafish, Danio rerio, Mammalia, Rattus, Vertebrata
Como citar
FEBS Journal, v. 275, n. 13, p. 3325-3334, 2008.