Electrogravimetric Analysis by Quartz-Crystal Microbalance on the Consumption of the Neurotransmitter Acetylcholine by Acetylcholinesterase
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Data
2012-12-01
Autores
Bueno, Paulo Roberto [UNESP]
Gonçalves, Luís M.
dos Santos, Fernanda C. [UNESP]
dos Santos, Marcio L. [UNESP]
Barros, Aquiles A.
Faria, Ronaldo C.
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Resumo
Electrogravimetric analysis was performed on the consumption of the neurotransmitter Acetylcholine (ACh) by Acetylcholinesterase (AChE) in situ and in real time. Michaelis-Menten assumption was achieved by using an enzyme micro-reactor in which the total enzyme was anchored in a quartz crystal microbalance chip (QCM-chip) with a strategically engineered self-assembled monolayer (SAM) of alkanethiols, which can prevent diffusion-controlled or spatially restricted kinetics. The real-time frequency changes indicated the rate of the products formation from enzymatic reaction. The QCM-chip was tested showing that it could demonstrate AChE inhibition by physostigmine. © 2013 Copyright Taylor and Francis Group, LLC.
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Acetyl cholinesterase, Acetylcholine, Neurotransmitter, Physostigmine, Quartz-crystal microbalance
Como citar
Analytical Letters, v. 46, n. 2, p. 258-265, 2012.