In vitro assessment of the cytotoxic, apoptotic, and mutagenic potentials of Isatin
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Data
2013-03-01
Autores
Cândido-Bacani, Priscila de Matos
Mori, Mateus Prates
Calvo, Tamara Regina [UNESP]
Vilegas, Wagner [UNESP]
Varanda, Eliana Aparecida [UNESP]
De Syllos Cólus, Ilce Mara
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Resumo
Isatin (1H-indole-2,3-dione) is a chemical found in various medicinal plant species and responsible for a broad spectrum of pharmacological and biological properties that may be beneficial to human health, as an anticonvulsant, antibacterial, antifungal, antiviral, and anticancer agent. The aim of the present study was to determine in vitro the cytotoxic, mutagenic, and apoptotic effects of isatin on CHO-K1 and HeLa cells using the MTT viability assay (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide), micronucleus (MN) test, apoptosis index, and nuclear division index (NDI). The 5 isatin concentrations evaluated in the mutagenicity and apoptosis tests were 0.5, 1, 5, 10, and 50 μM, selected through a preliminary MTT assay. Positive (doxorubicin, DXR) and negative (phosphate buffered saline, PBS) control groups were also included in the analysis. Isatin did not exert a mutagenic effect on CHO-K1 after 3 and 24 h of treatment or on HeLa cells after 24 h. However, 10 and 50 μM concentrations inhibited cell proliferation and promoted apoptosis in both CHO-K1 and HeLa cells. Data indicate that the cytotoxic, apoptotic, and antiproliferative effects of isatin were concentration independent and cell line independent. The authors thank Profa Dra Eiko Nakagawa Itano for the use the spectrophotometer and the Conselho Nacional para o Desenvolvimento Científico e Tecnológico for master's scholarships to P. M. Cândido-Bacani and grants to T. R. Calvo, W. Vilegas, E. A. Varanda and I. M. S. Cólus. The Conselho Nacional para o Desenvolvimento Científico e Tecnológico provided funding for this study. © 2013 Taylor & Francis Group, LLC.
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3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide, antineoplastic agent, doxorubicin, isatin, animal cell, antineoplastic activity, apoptosis, apoptosis index, cells, controlled study, cytotoxicity, HeLa cell, human, human cell, in vitro study, micronucleus test, mutagenicity, nonhuman, nuclear division index, priority journal, Animals, Apoptosis, Cell Division, Cell Nucleus, Cell Survival, CHO Cells, Cricetinae, Cricetulus, DNA, Neoplasm, Dose-Response Relationship, Drug, Female, HeLa Cells, Humans, Isatin, Micronuclei, Chromosome-Defective, Micronucleus Tests, Mutagens, Plant Extracts, Plants, Medicinal, Tetrazolium Salts, Thiazoles
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Journal of Toxicology and Environmental Health - Part A: Current Issues, v. 76, n. 6, p. 354-362, 2013.