Análise das células inflamatórias e expressão da proteína anti-inflamatória anexina A1 em pacientes com endometriose
Data
2013-02-28
Autores
Paula Junior, Rubens de [UNESP]
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ISSN da Revista
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
A endometriose é uma doença inflamatória crônica, de etiologia multifatorial caracterizada pela implantação e crescimento do endométrio fora da cavidade uterina (endométrio ectópico). Nas lesões de endometriose, os mastócitos aparecem em elevado número, no entanto, poucos estudos investigaram seus mediadores inflamatórios na patogênese dessa doença. Entre os mediadores dos mastócitos ressaltamos a anexina A1 (ANXA1), proteína de 37 kDa com múltiplas ações biológicas como inibição da transmigração de leucócitos, proliferação celular e apoptose. Tais efeitos são mediados por receptores para peptídeos formilados (FPRs), especialmente FPR1. Assim, o objetivo desse trabalho foi investigar o papel das células inflamatórias, particularmente a ativação e heterogeneidade dos mastócitos, sua correlação com a expressão e o mecanismo de ação da ANXA1 em biópsias de endométrios eutópicos (controle, n=10) e ectópicos (endometriose de parede abdominal, n=18). As amostras de endométrio ectópico foram coletadas entre julho de 2003 e janeiro de 2012, incluídas em parafina e cedidas pelo Departamento de Patologia da FAMERP. As biópsias de endométrios eutópicos foram coletadas de pacientes sem quadro clínico de endometriose, entre junho de 2011 e junho de 2012, com a colaboração do Departamento de Ginecologia e Obstetrícia da FAMERP. Essas biópsias foram processadas para: (i) análises histopatológicas, (ii) quantificação de células inflamatórias (neutrófilos e mastócitos) e (iii) imuno-histoquímica utilizando anticorpos específicos para estudo da heterogeneidade dos mastócitos (triptase e quimase), da expressão da ANXA1 e do receptor FPR1 nos tecidos. A análise histopatológica das lesões de endometriose mostrou...
Endometriosis is a chronic inflammatory disease of multifactorial etiology, characterized by implantation and growth of endometrium outside the uterine cavity (ectopic endometrium). Endometriotic lesions presented a high number of mast cells, however, few studies have investigated their inflammatory mediators in the pathogenesis of this disease. Among these mediators we emphasize annexin A1 (ANXA1), a 37 kDa protein with multiple biological actions like inhibition of leukocyte transmigration, cellular proliferation and apoptosis. Such effects are mediated by formyl peptide receptors (FPRs), especially FPR1. Thus, the purpose of this study was to investigate the role of inflammatory cells, particularly the activation and heterogeneity of mast cells, and its correlation with expression and mechanism of action of ANXA1 in biopsies of eutopic (control, n = 10) and ectopic endometrium (abdominal wall endometriosis; n = 18). The samples of ectopic endometrium were collected between July 2003 and January 2012, embedded in paraffin and provided by the Department of Pathology of FAMERP. Biopsies of eutopic endometrium were collected from patients without clinical features of endometriosis, between June 2011 and June 2012, with the contribution of the Department of Obstetrics and Gynecology of FAMERP. These biopsies were processed for: (i) histopathology, (ii) the quantification of inflammatory cells (neutrophils and mast cells) and (iii) immunohistochemistry using specific antibodies to study the heterogeneity of mast cells (chymase and tryptase), and expression of ANXA1 and FPR1 receptor in tissues. Histopathological analysis of endometriotic lesions showed two morphological patterns: glandular pattern of mixed differentiation (presence of differentiated glands with simple prismatic epithelium and... (Complete abstract click electronic access below)
Endometriosis is a chronic inflammatory disease of multifactorial etiology, characterized by implantation and growth of endometrium outside the uterine cavity (ectopic endometrium). Endometriotic lesions presented a high number of mast cells, however, few studies have investigated their inflammatory mediators in the pathogenesis of this disease. Among these mediators we emphasize annexin A1 (ANXA1), a 37 kDa protein with multiple biological actions like inhibition of leukocyte transmigration, cellular proliferation and apoptosis. Such effects are mediated by formyl peptide receptors (FPRs), especially FPR1. Thus, the purpose of this study was to investigate the role of inflammatory cells, particularly the activation and heterogeneity of mast cells, and its correlation with expression and mechanism of action of ANXA1 in biopsies of eutopic (control, n = 10) and ectopic endometrium (abdominal wall endometriosis; n = 18). The samples of ectopic endometrium were collected between July 2003 and January 2012, embedded in paraffin and provided by the Department of Pathology of FAMERP. Biopsies of eutopic endometrium were collected from patients without clinical features of endometriosis, between June 2011 and June 2012, with the contribution of the Department of Obstetrics and Gynecology of FAMERP. These biopsies were processed for: (i) histopathology, (ii) the quantification of inflammatory cells (neutrophils and mast cells) and (iii) immunohistochemistry using specific antibodies to study the heterogeneity of mast cells (chymase and tryptase), and expression of ANXA1 and FPR1 receptor in tissues. Histopathological analysis of endometriotic lesions showed two morphological patterns: glandular pattern of mixed differentiation (presence of differentiated glands with simple prismatic epithelium and... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Proteínas - Estrutura, Endometriose, Endometrio - Inflamação, Anexina A1, Agentes anti-inflamatorios, Mastocitos, Proteínas - Estrutura
Como citar
PAULA JUNIOR, Rubens de. Análise das células inflamatórias e expressão da proteína anti-inflamatória anexina A1 em pacientes com endometriose. 2013. 68 f. Dissertação (mestrado) - Universidade Estadual Paulista Julio de Mesquita Filho, Instituto de Biociências, Letras e Ciências Exatas de São José do Rio Preto, 2013.