Salmonella profile in chickens determined by real-time polymerase chain reaction and bacteriology from years 2000 to 2003 in Turkey
Loading...
Date
2005-04
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor & Francis
Abstract
From years 2000 to 2003, Salmonella was investigated from a total of 1785 samples comprised of chicken intestinal samples, cloacal swabs, drag swabs, litter samples and chick dust samples collected from 191 poultry breeding flocks belonging to 15 different chicken breeding stock companies in the Marmara region, Turkey by a SYBR green-based real-time polymerase chain reaction (SGBRT-PCR), by a probe-specific real-time polymerase chain reaction (PSRT-PCR) and by standardized bacteriology as described in the manual of National Poultry Improvement Plan and Auxillary Provisions, United States Department of Agriculture. Between January 2000 and July 2001, Salmonella was detected at the rates of 5.87% and 4.10% out of a total of 1242 samples by SGBRT-PCR and bacteriology, respectively. From July 2001 until December 2003, Salmonella was found at rates of 11.42% and 5.52% from a total of 543 samples by PSRT-PCR and bacteriology, respectively. The dominant Salmonella serovar was determined as Salmonella enterica subsp. enterica Serovar Enteritidis ( S. Enteritidis), while serogroup C1 and C2 in 2001 and serogroup E1 in 2002 were isolated as additional serovars. As a conclusion, S. Enteritidis seems to be the major problem in poultry breeding flocks in Turkey, and both of the real-time polymerase chain reaction methods were found more sensitive than standard bacteriology for the detection of Salmonella from poultry samples.
Description
Keywords
Tetrathionate broth enrichment, Enterica serotype enteritidis, Environmental-samples, Serogrup-D, PCR, Poultry, Typhimurium, Feces, Identification, Serovars, Veterinary sciences, Aves, Enteritidis, Gallus gallus, Marmara, Meleagris gallopavo, Salmonella, Salmonella enterica, Salmonella enterica subsp. enterica serovar enteritidis, Salmonella enteritidis
Citation
Eyigör, O. vd. (2005). "Salmonella profile in chickens determined by real-time polymerase chain reaction and bacteriology from years 2000 to 2003 in Turkey". Avian Pathology, 34(2), 101-105.