The combinatorial peptide ligand library (CPLL) in association with mass spectrometry (MS) can greatly enhance the dynamic range of the analysis of low and very low-abundance proteins constituting the vast majority of species in any sample. When compared with untreated samples, the increment in detection of low-abundance species appears to be at least four-fold. Recently, CPLL have been challenged, however it has been clearly demonstrated in the evaluated paper that the protocols for elution of the captured polypeptides make the difference. Therefore, the solid-phase ligand library made of hexapeptides, remains a promising unique tool for biomarker discovery.

"Proteomineering": has the mine been excavated?

PANFOLI, ISABELLA;M. Bruschi;RAVERA, SILVIA;
2011-01-01

Abstract

The combinatorial peptide ligand library (CPLL) in association with mass spectrometry (MS) can greatly enhance the dynamic range of the analysis of low and very low-abundance proteins constituting the vast majority of species in any sample. When compared with untreated samples, the increment in detection of low-abundance species appears to be at least four-fold. Recently, CPLL have been challenged, however it has been clearly demonstrated in the evaluated paper that the protocols for elution of the captured polypeptides make the difference. Therefore, the solid-phase ligand library made of hexapeptides, remains a promising unique tool for biomarker discovery.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11567/392072
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