A proteomic approach was used to identify proteins differentially expressed in the plasma of mice following alternate day fasting. Male mice (C57Bl6, 9 months old) were randomly assigned to ad libitum (AL, n = 10) and calorie-restricted (IF, n = 10, AL on alternate day basis) groups for 4 weeks. After staining, the gels were imaged and differential protein expression patterns were interrogated using image analysis software. Spots showing a different expression level were identified through a comparison with 2D maps found in databases officially recognized (ExPASy). Master gels of AL and IF mice exhibited slightly different 2-DE patterns as only 14 spots out of 500 appeared differentially expressed: 12 spots were downregulated and identified as apolipoprotein E (APO-E), haptoglobin (HPT), alpha-1-antitrypsin (A1AT), prothrombin, and serum amyloid P component (SPA), whereas two spots were unidentified. In conclusion 4 weeks of alternate feeding results in a slight perturbation of the host plasma proteome. Of particular interest is the reduced plasma content in APO-E and acute-phase proteins HPT, A1AT and SPA that confirms a powerful anti-inflammatory effect of this dietary regimen. Keywords Alternate day fastin
Proteomic analysis of plasma after 4 weeks of intermittent fasting in mice
MASCARO, ANNA;D'ANTONA, GIUSEPPE
2013-01-01
Abstract
A proteomic approach was used to identify proteins differentially expressed in the plasma of mice following alternate day fasting. Male mice (C57Bl6, 9 months old) were randomly assigned to ad libitum (AL, n = 10) and calorie-restricted (IF, n = 10, AL on alternate day basis) groups for 4 weeks. After staining, the gels were imaged and differential protein expression patterns were interrogated using image analysis software. Spots showing a different expression level were identified through a comparison with 2D maps found in databases officially recognized (ExPASy). Master gels of AL and IF mice exhibited slightly different 2-DE patterns as only 14 spots out of 500 appeared differentially expressed: 12 spots were downregulated and identified as apolipoprotein E (APO-E), haptoglobin (HPT), alpha-1-antitrypsin (A1AT), prothrombin, and serum amyloid P component (SPA), whereas two spots were unidentified. In conclusion 4 weeks of alternate feeding results in a slight perturbation of the host plasma proteome. Of particular interest is the reduced plasma content in APO-E and acute-phase proteins HPT, A1AT and SPA that confirms a powerful anti-inflammatory effect of this dietary regimen. Keywords Alternate day fastinI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
