Sera from 20 patients with antiphospholipid syndrome (APS), primary or secondary to systemic lupus erythematosus (SLE), or with SLE, were assayed by immunoblot analysis for anti-beta(2)-glycoprotein I antibodies (a beta(2)-GPI), and by indirect immunofluorescence (IIF) technique for reactivity with astrocyte and neuron cell lines and with histological sections of human brain biopsies and monkey cerebellum. Six sera from healthy donors were studied as a control. Eleven out of the 20 patient sera contained a beta(2)-GPI and were immunoreactive with astrocytes and neurons, both in culture and in the histological sections, and with the endotheliocytes of the microvessels present in the histological sections. Cell localization and the pattern of immune reaction were similar to those obtained with a monoclonal antibody a beta(2)-GPI. Eight of the remaining patient sera, found a beta(2)-GPI(-), did not react with the nervous substrates (and the control sera), while one exhibited immunoreactivity analogous to the a beta(2)-GPI(+) sera. The interference of anticardiolipin antibodies (aCL) in the immunoreactivity with the nervous substrates was excluded since aCL were present in all patient sera and no immune reaction was observed in the histological sections incubated with a monoclonal aCL. Therefore, the binding of a beta(2)-GPI from patients to cells of the central nervous system (CNS) occurs independently from aCL. This issue may be relevant to further evaluate the potential pathogenetic role of a beta(2)-GPI in the CNS damage of APS-like conditions. (C) 1998 Academic Press
Serum anti-beta(2)-glycoprotein I antibodies from patients with antiphospholipid antibody syndrome bind central nervous system cells / Caronti, Brunella; Caterina, Calderaro; Alessandri, Cristiano; Conti, Fabrizio; Raffaella, Tinghino; Pini, Carlo; Palladini, Guido; Valesini, Guido. - In: JOURNAL OF AUTOIMMUNITY. - ISSN 0896-8411. - 11:5(1998), pp. 425-429. (Intervento presentato al convegno 5th International Conference on SLE tenutosi a CANCUN, MEXICO nel APR 20-25, 1998) [10.1006/jaut.1998.0214].
Serum anti-beta(2)-glycoprotein I antibodies from patients with antiphospholipid antibody syndrome bind central nervous system cells
CARONTI, Brunella;ALESSANDRI, cristiano;CONTI, FABRIZIO;PINI, CARLO;PALLADINI, Guido;VALESINI, Guido
1998
Abstract
Sera from 20 patients with antiphospholipid syndrome (APS), primary or secondary to systemic lupus erythematosus (SLE), or with SLE, were assayed by immunoblot analysis for anti-beta(2)-glycoprotein I antibodies (a beta(2)-GPI), and by indirect immunofluorescence (IIF) technique for reactivity with astrocyte and neuron cell lines and with histological sections of human brain biopsies and monkey cerebellum. Six sera from healthy donors were studied as a control. Eleven out of the 20 patient sera contained a beta(2)-GPI and were immunoreactive with astrocytes and neurons, both in culture and in the histological sections, and with the endotheliocytes of the microvessels present in the histological sections. Cell localization and the pattern of immune reaction were similar to those obtained with a monoclonal antibody a beta(2)-GPI. Eight of the remaining patient sera, found a beta(2)-GPI(-), did not react with the nervous substrates (and the control sera), while one exhibited immunoreactivity analogous to the a beta(2)-GPI(+) sera. The interference of anticardiolipin antibodies (aCL) in the immunoreactivity with the nervous substrates was excluded since aCL were present in all patient sera and no immune reaction was observed in the histological sections incubated with a monoclonal aCL. Therefore, the binding of a beta(2)-GPI from patients to cells of the central nervous system (CNS) occurs independently from aCL. This issue may be relevant to further evaluate the potential pathogenetic role of a beta(2)-GPI in the CNS damage of APS-like conditions. (C) 1998 Academic PressI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.