An anal. procedure for the detn. of natural steroids and their conjugates was developed and applied to bovine and human urine. Fifteen compds. (free and conjugated) after extn. by solid-phase were identified by liq. chromatog.-tandem mass spectrometry (LC-MS-MS) in multiple reaction monitoring (MRM). Free and conjugated steroids were detected all together in the same chromatog. anal. resp. in pos. and neg. ionization. This procedure reveals to be time-saving, because it overcomes the need of further sample pre-treatment steps, such as enzymic hydrolysis and derivatization. The recovery for each compd. was around 80%. Limit of detection of each steroid free or conjugated has been evaluated.
Development of a liquid chromatography-tandem mass spectrometry method for the identification of natural androgen steroids and their conjugates in urine samples / Buiarelli, Francesca; Coccioli, Franco; Merolle, M; Neri, B; Terracciano, A.. - In: ANALYTICA CHIMICA ACTA. - ISSN 0003-2670. - ELETTRONICO. - 526:(2004), pp. 113-120. [10.1016/j.aca.2004.09.068]
Development of a liquid chromatography-tandem mass spectrometry method for the identification of natural androgen steroids and their conjugates in urine samples
BUIARELLI, Francesca;COCCIOLI, Franco;
2004
Abstract
An anal. procedure for the detn. of natural steroids and their conjugates was developed and applied to bovine and human urine. Fifteen compds. (free and conjugated) after extn. by solid-phase were identified by liq. chromatog.-tandem mass spectrometry (LC-MS-MS) in multiple reaction monitoring (MRM). Free and conjugated steroids were detected all together in the same chromatog. anal. resp. in pos. and neg. ionization. This procedure reveals to be time-saving, because it overcomes the need of further sample pre-treatment steps, such as enzymic hydrolysis and derivatization. The recovery for each compd. was around 80%. Limit of detection of each steroid free or conjugated has been evaluated.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
