The ability of quaternized polyamines (poly-N-alkyl-4-vinylpyridinium bromides possessing a number, m, of methylene groups in the N-alkyl substituent or a degree of alkylation, beta, and n,n-ionene bromides) to suppress the thermoaggregation of glyceraldehyde-3-phosphate dehydrogenase increased in the order m = 1 < 3 < 5, beta = 95 < 85 < 70 << 45 < 35 < 20 and n = 3 < 6 < 10, which agrees well with the increase, in the same order, in the hydrophobicity of the chains. Complexing suppressed thermoaggregation, but not thermodenaturation of the enzyme, which was even encouraged by the polycations and occurred at room temperature when the most efficient suppressor (with beta = 20) was used. The adverse effect was reduced by the addition of sodium chloride which destroyed the complex and resulted in a noticeable reactivation.
Interaction of polyelectrolytes with proteins, 3 Influence of complexing polycations on the thermoaggregation of oligomeric enzymes / Irina N., Shalova; Irina N., Naletova; Saso, Luciano; Vladimir I., Muronetz; Vladimir A., Izumrudov. - In: MACROMOLECULAR BIOSCIENCE. - ISSN 1616-5187. - 7:7(2007), pp. 929-939. [10.1002/mabi.200700052]
Interaction of polyelectrolytes with proteins, 3 Influence of complexing polycations on the thermoaggregation of oligomeric enzymes
SASO, Luciano;
2007
Abstract
The ability of quaternized polyamines (poly-N-alkyl-4-vinylpyridinium bromides possessing a number, m, of methylene groups in the N-alkyl substituent or a degree of alkylation, beta, and n,n-ionene bromides) to suppress the thermoaggregation of glyceraldehyde-3-phosphate dehydrogenase increased in the order m = 1 < 3 < 5, beta = 95 < 85 < 70 << 45 < 35 < 20 and n = 3 < 6 < 10, which agrees well with the increase, in the same order, in the hydrophobicity of the chains. Complexing suppressed thermoaggregation, but not thermodenaturation of the enzyme, which was even encouraged by the polycations and occurred at room temperature when the most efficient suppressor (with beta = 20) was used. The adverse effect was reduced by the addition of sodium chloride which destroyed the complex and resulted in a noticeable reactivation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.