Short chain alkyl monoglucosides (octyl and nonyl-β-glucoside) are soluble in aqueous solutions of ammonium sulfate up to 4 M ionic strength. In combination with PEG of molecular weight from 3350 to 10000, two phase transitions can be observed while increasing temperature, with lower and upper two-phase regions separated by a single isotropic solution. The one-phase region narrows on increasing the concentration of polymer and surfactant, and for PEG 8000 and at high ratios of polymer to glucoside, becomes a small channel where solutions are sensitive to shear. In solutions with octyl-β-glucoside the lower two-phase body is shifted to higher concentrations than the one in solutions with nonyl-β-glucoside, while the upper two-phase body does not vary significantly. The implications of these patterns of phase behavior for membrane protein crystallization will be discussed.
Phase behavior of short chain alkyl monoglucosides in solutions with PEG and ammonium sulfate / Santonicola, Mariagabriella; M. A., Yocum; A. M., Lenhoff; E. W., Kaler. - In: ABSTRACTS OF PAPERS - AMERICAN CHEMICAL SOCIETY. - ISSN 0065-7727. - ELETTRONICO. - 229:(2005), pp. U716-U716. (Intervento presentato al convegno 229th National Meeting of the American-Chemical-Society tenutosi a San Diego, CA nel MAR 13-17, 2005).
Phase behavior of short chain alkyl monoglucosides in solutions with PEG and ammonium sulfate.
SANTONICOLA, MARIAGABRIELLA;
2005
Abstract
Short chain alkyl monoglucosides (octyl and nonyl-β-glucoside) are soluble in aqueous solutions of ammonium sulfate up to 4 M ionic strength. In combination with PEG of molecular weight from 3350 to 10000, two phase transitions can be observed while increasing temperature, with lower and upper two-phase regions separated by a single isotropic solution. The one-phase region narrows on increasing the concentration of polymer and surfactant, and for PEG 8000 and at high ratios of polymer to glucoside, becomes a small channel where solutions are sensitive to shear. In solutions with octyl-β-glucoside the lower two-phase body is shifted to higher concentrations than the one in solutions with nonyl-β-glucoside, while the upper two-phase body does not vary significantly. The implications of these patterns of phase behavior for membrane protein crystallization will be discussed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
