A number of morbillivirus infection epidemics causing mass mortality have been reported in recent years in several free-living sea mammal species around the world. Their causative agents, at least three different morbilliviruses, were unknown in aquatic mammals before 1988. Affected animals developed severe microscopic lesions mainly involving the lung, brain and lymph nodes. All the above, along with a number of other tissues, harbour large amounts of viral antigen, which may be demonstrated therein by means of appropriate immunohistochemicalstaining techniques. An immunohistochemistryprotocol, based upon utilization of monoclonal antibodies recognizing different epitopes of one of the viruses, phocine distempervirus, or against a closely related agent, canine distemper virus, may be successfullyused for both diagnostic and research purposes. This article reports the immunohistochemicaltechnique and its results.[...]
Immunohistochemistry and Morbillivirus infections in sea mammals
DI GUARDO, Giovanni;Della Salda L;MARRUCHELLA, GIUSEPPE;
2004-01-01
Abstract
A number of morbillivirus infection epidemics causing mass mortality have been reported in recent years in several free-living sea mammal species around the world. Their causative agents, at least three different morbilliviruses, were unknown in aquatic mammals before 1988. Affected animals developed severe microscopic lesions mainly involving the lung, brain and lymph nodes. All the above, along with a number of other tissues, harbour large amounts of viral antigen, which may be demonstrated therein by means of appropriate immunohistochemicalstaining techniques. An immunohistochemistryprotocol, based upon utilization of monoclonal antibodies recognizing different epitopes of one of the viruses, phocine distempervirus, or against a closely related agent, canine distemper virus, may be successfullyused for both diagnostic and research purposes. This article reports the immunohistochemicaltechnique and its results.[...]I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
