Human Asf1 and CAF-1 interact and synergize in a repair-coupled nucleosome 
assembly pathway

Mello, J. A.; Sillje, H. H. W.; Roche, D. M. J.; Kirschner, D. B.; Nigg, E. A.; Almouzni, G.

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Human Asf1 and CAF-1 interact and synergize in a repair-coupled nucleosome assembly pathway

MPS-Authors

/persons/resource/persons78459

Nigg, E. A.
Former Research Groups, Max Planck Institute of Biochemistry, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Mello, J. A., Sillje, H. H. W., Roche, D. M. J., Kirschner, D. B., Nigg, E. A., & Almouzni, G. (2002). Human Asf1 and CAF-1 interact and synergize in a repair-coupled nucleosome assembly pathway. EMBO Reports, 3(4), 329-334.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-6F78-9

Abstract

The efficient assembly of newly replicated and repaired DNA into chromatin is essential for proper genome function. Based on genetic studies in Saccharomyces cerevisiae, the histone chaperone anti-silencing function 1 (Asf1) has been implicated in the DNA repair response. Here, the human homologs are shown to function synergistically with human CAF-1 to assemble nucleosomes during nucleotide excision repair in vitro. Furthermore, we demonstrate that hAsf1 proteins can interact directly with the p60 subunit of hCAF-1. In contrast to hCAF-1 p60, the nuclear hAsf1 proteins are not significantly associated with chromatin in cells before or after the induction of DNA damage, nor specifically recruited to damaged DNA during repair in a bead-linked DNA assay. A model is proposed in which the synergism between hAsf1 and CAF-1 for nucleosome formation during DNA repair is achieved through a transient physical interaction allowing histone delivery from Asf1 to CAF-1.