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Journal Article

Analysis of gene function in somatic mammalian cells using small interfering RNAs

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Elbashir,  S. M.
Research Group of Combinatorical Biochemistry, MPI for biophysical chemistry, Max Planck Society;

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Harborth,  J.
Department of Biochemistry and Cell Biology, MPI for biophysical chemistry, Max Planck Society;

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Weber,  K.
Department of Biochemistry and Cell Biology, MPI for biophysical chemistry, Max Planck Society;

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Tuschl,  T.
Research Group of Combinatorical Biochemistry, MPI for biophysical chemistry, Max Planck Society;

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Elbashir, S. M., Harborth, J., Weber, K., & Tuschl, T. (2002). Analysis of gene function in somatic mammalian cells using small interfering RNAs. Methods, 26(2), 199-213. Retrieved from http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WN5-45WGJF8-F-1P&_cdi=6953&_user=38661&_pii=S1046202302000233&_origin=search&_coverDate=02%2F28%2F2002&_sk=999739997&view=c&wchp=dGLbVtz-zSkWA&md5=c94da4baa1bbe2a59c130b0ea900a490&ie=/sdarticle.pdf.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0012-F450-1
Abstract
RNA interference (RNAi) is a highly conserved gene silencing mechanism that uses double-stranded RNA (dsRNA) as a signal to trigger the degradation of homologous mRNA. The mediators of sequence-specific mRNA degradation are 21- to 23-nt small interfering RNAs (siRNAs) generated by ribonuclease III cleavage from longer dsRNAs. Twenty-one-nuclcotide siRNA duplexes trigger specific gene silencing in mammalian somatic cells without activation of the unspecific interferon response. Here we provide a collection of protocols for siRNA-mediated knockdown of mammalian gene expression. Because of the robustness of the siRNA knockdown technology. genomewide analysis of human gene function in cultured cells has now become possible. (C) 2002 Elsevier Science (USA). All rights reserved.