A hybrid approach to the 3D high precision position measurement and particle 
tracking in human cell nuclei

Cavallo, A.; Solovei, I.; Schermeleh, L.; Heintzmann, R.; Cremer, T.; Cremer, C.

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A hybrid approach to the 3D high precision position measurement and particle tracking in human cell nuclei

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Heintzmann, R.
Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society;

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Citation

Cavallo, A., Solovei, I., Schermeleh, L., Heintzmann, R., Cremer, T., & Cremer, C. (2002). A hybrid approach to the 3D high precision position measurement and particle tracking in human cell nuclei. Real-Time Imaging, 8, 467-473. Retrieved from http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WPR-47C4CC5-B-1&_cdi=6997&_user=38661&_pii=S1077201402902895&_origin=search&_coverDate=12%2F31%2F2002&_sk=999919993&view=c&wchp=dGLbVzb-zSkzk&md5=79d3f86853557cbb7a9b03f8903eafca&ie=/sdarticle.pdf.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0012-F4E0-0

Abstract

In this report, we present a detection and tracking system for fluorescent particles inside a cell nucleus. We present the application of this system to a real biological problem where the same human cell nucleus is scanned in three different states: living, fixed and after a treatment used for fluorescence in situ hybridization (FISH). The change in distances between internal subchromosomal structures was analyzed.