Neuroprotection by rAAV-mediated gene transfer of bone morphogenic protein 7

Heinonen, Ann-Marie; Rahman, Mahbubur; Dogbevia, Godwin; Jakobi, Hannah; Wölfl, Stefan; Sprengel, Rolf; Schwaninger, Markus

doi:10.1186/1471-2202-15-38

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Neuroprotection by rAAV-mediated gene transfer of bone morphogenic protein 7

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Heinonen, Ann-Marie
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Dogbevia, Godwin
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Sprengel, Rolf
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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http://dx.doi.org/10.1186/1471-2202-15-38
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Citation

Heinonen, A.-M., Rahman, M., Dogbevia, G., Jakobi, H., Wölfl, S., Sprengel, R., et al. (2014). Neuroprotection by rAAV-mediated gene transfer of bone morphogenic protein 7. BMC Neuroscience, 15(1), 38-49. doi:10.1186/1471-2202-15-38.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0019-8F88-8

Abstract

Bone morphogenic proteins (BMPs) promote the survival of neurons, suggesting a therapeutic application of BMPs in the treatment of acute and chronic neurodegenerative disorders. However, the application of recombinant BMPs in vivo is limited by their short half-life. To provide a continuous supply for functionally active BMPs, we expressed BMP7, BMP2 and the BMP inhibitor Noggin under the control of rAAV vectors in vivo. For visual control of rAAV-mediated BMP (v-BMP) expression we fused the secreted morphogenic polypeptides and the fluorescent reporter protein Venus via the ‘ribosomal skip’ promoting 2A peptide-bridge.