Transactivation of the human c-myc gene by c-Myb.

Zobel, A.; Kalkbrenner, F.; Vorbrüggen, G.; Mölling, K.

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Transactivation of the human c-myc gene by c-Myb.

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Vorbrüggen, G.
Research Group of Molecular Cell Dynamics, MPI for biophysical chemistry, Max Planck Society;

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http://linkinghub.elsevier.com/retrieve/pii/0006-291X(92)90805-U
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Citation

Zobel, A., Kalkbrenner, F., Vorbrüggen, G., & Mölling, K. (1992). Transactivation of the human c-myc gene by c-Myb. Biochemical and Biophysical Research Communications, 186(2), 715-722.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0029-294C-D

Abstract

We analyse the contribution of six Myb-binding sites in the upstream c-myc sequences to transactivation by co-transfection assays. Surprisingly, deletion of the six Myb-binding sites did not influence the transactivation of c-myc by c-Myb protein. Instead, the strongest transactivation was observed with a c-myc reporter plasmid which contains only 450 bp of exon 1 including the c-myc promoter P2. An exchange of the DNA binding domain of c-Myb by that of GAL4 led only to small transactivation effects indicating that the DNA binding domain of c-Myb is essential for transactivation of the c-myc gene. These results suggest either an indirect transactivation mechanism of the c-myc gene by c-Myb proteins or a role of the DNA binding domain for additional effects than DNA binding.