English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

TakeTwo: an indexing algorithm suited to still images with known crystal parameters

MPS-Authors
/persons/resource/persons197908

Müller-Werkmeister,  Henrike
Department of Biochemistry, University of Toronto, King’s College Circle, Toronto, ON M5S 1A8, Canada;
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Departments of Physics and Chemistry, University of Toronto, 80 St George Street, Toronto, ON M5S 1H6, Canada;

/persons/resource/persons136024

Miller,  R. J. Dwayne
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Departments of Physics and Chemistry, University of Toronto, 80 St George Street, Toronto, ON M5S 1H6, Canada;
Hamburg Centre for Ultrafast Imaging, University of Hamburg, Hamburg, Germany;

External Resource

http://dx.doi.org/10.1107/S2059798316010706
(Publisher version)

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)

rr5128.pdf
(Publisher version), 2MB

Supplementary Material (public)
There is no public supplementary material available
Citation

Ginn, H. M., Roedig, P., Kuo, A., Evans, G., Sauter, N. K., Ernst, O., et al. (2016). TakeTwo: an indexing algorithm suited to still images with known crystal parameters. Acta Crystallographica Section D: Structural Biology, 72(8), 956-965. doi:10.1107/S2059798316010706.


Cite as: https://hdl.handle.net/11858/00-001M-0000-002B-1D2B-7
Abstract
The indexing methods currently used for serial femtosecond crystallography were originally developed for experiments in which crystals are rotated in the X-ray beam, providing significant three-dimensional information. On the other hand, shots from both X-ray free-electron lasers and serial synchrotron crystallo­graphy experiments are still images, in which the few three-dimensional data available arise only from the curvature of the Ewald sphere. Traditional synchrotron crystallography methods are thus less well suited to still image data processing. Here, a new indexing method is presented with the aim of maximizing information use from a still image given the known unit-cell dimensions and space group. Efficacy for cubic, hexagonal and orthorhombic space groups is shown, and for those showing some evidence of diffraction the indexing rate ranged from 90% (hexagonal space group) to 151% (cubic space group). Here, the indexing rate refers to the number of lattices indexed per image.