Hereditary Cerebral Hemorrhage with Amyloidosis-Dutch type (HCHWA-D) is an autosomal dominant hereditary disease caused by a point mutation in exon 17 of the APP gene. We generated human induced pluripotent stem cells (hiPSCs) from a symptomatic HCHWA-D patient by using non-integrating Sendai virus (SeV). The newly generated hiPSCs express all pluripotency markers, have a normal karyotype, carry the Dutch mutation, can differentiate in the three germ layers in vitro and are SeV free.

doi.org/10.1016/j.scr.2018.101359, hdl.handle.net/1765/113801
Stem Cell Research
Erasmus MC: University Medical Center Rotterdam

Daoutsali, E. (Elena), Buijsen, R., van de Pas, S., Jong, A. (Anke 't), Mikkers, H., Brands, T. (Tom), … van Roon-Mom, W. (2019). Generation of 3 human induced pluripotent stem cell lines LUMCi005-A, B and C from a Hereditary Cerebral Hemorrhage with Amyloidosis-Dutch type patient. Stem Cell Research, 34. doi:10.1016/j.scr.2018.101359