Title:

Proteomic Analyses of Human Fetal Atria and Ventricles

Author: Lu, Zhen Qi
Department: Physiology
Issue Date: Nov-2014
Abstract (summary): In this study we carried out a mass spectrometry-based proteome analysis of human fetal atria and ventricles. Heart protein lysates were analyzed on the Q-Exactive mass spectrometer in biological triplicates. Protein identification using MaxQuant yielded a total of 2,754 atrial (91%) and 2,825 ventricular protein groups (83%) in at least 2 of the 3 runs with 2 unique peptides. Statistical analyses using fold-enrichment (>2) and p-values (0.05) selected high confidence chamber enriched atrial (134) and ventricular (81) protein groups. Several previously characterised cardiac chamber-enriched proteins were identified in this study including atrial isoform of myosin light chain 2 (MYL7), atrial natriuretic peptide (NPPA), and connexin 40 (GJA5) for atria, and ventricular isoforms of myosin light chains (MYL2 and MYL3), myosin heavy chain 7 (MYH7), and connexin 43 (GJA1) for ventricle. Our data was compared to in-house generated and publicly available human microarrays, several human cardiac proteomes, and phenotype ontology databases.
Content Type: Thesis

Permanent link

https://hdl.handle.net/1807/74628

Items in TSpace are protected by copyright, with all rights reserved, unless otherwise indicated.