Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/42038
Título: | Investigation of Dendrimer-based nanoparticles cellular uptake and cell tracking in a semiautomated microfluidic platform |
Autor(es): | Carvalho, Mariana Rodrigues Maia, F. Raquel Reis, R. L. Oliveira, J. M. |
Palavras-chave: | Cancer Microfluidic Nanoparticles Extracellular matrix Biomaterial |
Data: | 2016 |
Citação: | Carvalho M. R., Maia F. R., Reis R. L., Oliveira J. M. Investigation of Dendrimer-based nanoparticles cellular uptake and cell tracking in a semi-automated microfluidic platform, Frontiers, doi:10.3389, 2016 |
Resumo(s): | A microfluidic device such as Kima Pump and Vena8 biochip is able to realize functions that are not easily imaginable in conventional biological analysis, such as highly parallel, sophisticated high-throughput analysis and single-cell analysis in a well-defined manner [1]. Cancer cell tracking within the microfluidic model will be achieved by grafting fluorescent label probe Fluorescein-5(6)-isothiocyanate (FITC) to dendrimer nanoparticles allowing cell visualization by immunofluorescent staining followed by fluorescence microscopy. In this study, synthesis and physicochemical characterization of Carboxymethyl-chitosan/poly(amidoamine) dendrimer nanoparticles (CMCht/PAMAM NPâ s) were performed[2].  Several cancer cell lines such as a HeLA (cervical carcinoma cell line), HTTC-116 (Colon Carcinoma) and Glioblastome cell line (GBM) were exposed to different concentrations of CMCht/PAMAM dendrimer nanoparticles over a period of 7d. After finding the adequate NP concentration, the internalization efficiency was tested, as well as cellular trafficking, in static and dynamic conditions (Kima Pump bioreactor). |
Tipo: | Resumo em ata de conferência |
URI: | https://hdl.handle.net/1822/42038 |
ISSN: | 2296-4185 |
Versão da editora: | http://www.frontiersin.org/10.3389/conf.FBIOE.2016.01.02034/event_abstract |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
18697-Frontiers _ Investigation of Dendrimer-based nanoparticles cellular uptake and cell tracking in a semi-automated microfluidic platform.pdf | 354,09 kB | Adobe PDF | Ver/Abrir |