Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/46857
Título: | Self-assembled hydrogel fiber bundles from oppositely charged polyelectrolytes mimic micro-/nanoscale hierarchy of collagen |
Autor(es): | Sant, Shilpa Coutinho, Daniela F. Gaharwar, Akhilesh K. Neves, N. M. Reis, R. L. Gomes, Manuela E. Khademhosseini, Ali |
Palavras-chave: | Bottom-up self-assembly Chitosan and gellan gum Collagen mimicking Hierarchical hydrogel fibers Polyelectrolyte Complexes |
Data: | Ago-2017 |
Editora: | Wiley-VCH Verlag |
Revista: | Advanced Functional Materials |
Citação: | Sant S., Coutinho D. F., Gaharwar A. K., Neves N. M., Reis R. L., Gomes M. E., Khademhosseini A. Self-Assembled Hydrogel Fiber Bundles from Oppositely Charged Polyelectrolytes Mimic Micro-/Nanoscale Hierarchy of Collagen, Adv. Funct. Mater., doi:10.1002/adfm.201606273, 2017 |
Resumo(s): | Fiber bundles are present in many tissues throughout the body. In most cases, collagen subunits spontaneously self-assemble into a fibrilar structure that provides ductility to bone and constitutes the basis of muscle contraction. Translating these natural architectural features into a biomimetic scaffold still remains a great challenge. Here, a simple strategy is proposed to engineer biomimetic fiber bundles that replicate the self-assembly and hierarchy of natural collagen fibers. The electrostatic interaction of methacrylated gellan gum with a countercharged chitosan polymer leads to the complexation of the polyelectrolytes. When directed through a polydimethylsiloxane channel, the polyelectrolytes form a hierarchical fibrous hydrogel demonstrating nanoscale periodic light/dark bands similar to D-periodic bands in native collagen and align parallel fibrils at microscale. Importantly, collagen-mimicking hydrogel fibers exhibit robust mechanical properties (MPa scale) at a single fiber bundle level and enable encapsulation of cells inside the fibers under cell-friendly mild conditions. Presence of carboxyl- (in gellan gum) or amino- (in chitosan) functionalities further enables controlled peptide functionalization such as Arginylglycylaspartic acid (RGD) for biochemical mimicry (cell adhesion sites) of native collagen. This biomimetic-aligned fibrous hydrogel system can potentially be used as a scaffold for tissue engineering as well as a drug/gene delivery vehicle. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/46857 |
DOI: | 10.1002/adfm.201606273 |
ISSN: | 1616-301X |
e-ISSN: | 1616-3028 |
Versão da editora: | http://onlinelibrary.wiley.com/doi/10.1002/adfm.201606273/full |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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19173-DanielaCoutinho_AdvancedFunctionalMaterials2017.pdf | 3,02 MB | Adobe PDF | Ver/Abrir |