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https://hdl.handle.net/1822/53713
Título: | Engineering patient-specific bioprinted constructs for treatment of degenerated intervertebral disc |
Autor(es): | Costa, João Bebiano Silva-Correia, Joana Ribeiro, Viviana Pinto da Silva Morais, Alain Oliveira, Joaquim M. Reis, R. L. |
Palavras-chave: | 3D printing Intervertebral disc Patient-specific Reverse engineering Silk fibroin/elastin bioink Tissue engineering |
Data: | 2019 |
Editora: | Elsevier |
Revista: | Materials Today Communications |
Resumo(s): | Lower back pain (LBP), which is strongly associated with intervertebral disc (IVD) degeneration, is one of the most frequently reported age- and work-related disorder in actual society, leading to a huge socio-economic impact worldwide. The current treatments have poor clinical outcomes and do not consider each patient needs. Thus, there is a growing interest in the potential of personalized cell-based tissue engineering (TE) approaches aimed to regenerate the damaged IVD and efficiently restore full disc function. In this work, a bioink composed by silk fibroin (SF) hydrogel combined with elastin was used to bioprint patient-specific substitutes mimicking IVD ultrastructure, in particular the outer region of the IVD (i.e. annulus fibrosus, AF). Following a reverse engineering approach, the proposed strategy makes use of a 3D model of AF obtained by semi-automatic morphological segmentation from magnetic resonance imaging dataset of human IVD. SF/elastin bioprinted scaffolds were characterized thoroughly in vitro, in terms of physico-chemical and biological performance. The bioprinted SF/elastin scaffolds were shown to possess structural and mechanical properties similar to the native AF and to support cell attachment and growth. Human adipose-derived stem cell cultured onto the SF/elastin bioprinted scaffolds were shown to adhere, proliferate and maintain metabolic activity and viability up to 21 days of culturing. The implantation of custom-made SF/elastin implants that best emulate a patient AF anatomy can potentially open up new personalized treatments for tackling IVD disorders by means of improving recovery time after surgery and helping to restore spine biofunctionality. |
Tipo: | Artigo |
Descrição: | Available online 14 February 2018 |
URI: | https://hdl.handle.net/1822/53713 |
DOI: | 10.1016/j.mtcomm.2018.01.011 |
ISSN: | 2352-4928 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Costa et al. 2018.pdf Acesso restrito! | 723,76 kB | Adobe PDF | Ver/Abrir |