High temporal-resolution view of transcription and chromatin states across distinct metabolic states in budding yeast
Date
2014
Authors
Kuang, Z.
Cai, L.
Zhang, Xuekui
Ji, H.
Tu, B.P.
Boeke, J.D.
Journal Title
Journal ISSN
Volume Title
Publisher
Nature Structural & Molecular Biology
Abstract
Under continuous, glucose-limited conditions, budding yeast exhibit robust metabolic cycles
associated with major oscillations of gene expression. How such fluctuations are linked to changes
in chromatin status is not well understood. Here we examine the correlated genome-wide
transcription and chromatin states across the yeast metabolic cycle at unprecedented temporal
resolution, revealing a “just-in-time supply chain” by which components from specific cellular
processes such as ribosome biogenesis become available in a highly coordinated manner. We
identify distinct chromatin and splicing patterns associated with different gene categories and
determine the relative timing of chromatin modifications to maximal transcription. There is
unexpected variation in the chromatin modification and expression relationship, with histone
acetylation peaks occurring with varying timing and “sharpness” relative to RNA expression both
within and between cycle phases. Chromatin modifier occupancy reveals subtly distinct spatial
and temporal patterns compared to the modifications themselves.
Description
Keywords
Citation
Kuang, Z., Cai, L., Zhang, X., Ji, H., Tu, B.P., Boeke, J.D. (2014). High temporalresolution view of transcription and chromatin states across distinct metabolic states in budding yeast. Nature Structural & Molecular Biology, 21, 854-863. https://doi.org/10.1038/nsmb.2881