Title:
Three-dimensional in Situ Temperature Measurement in Microsystems Using Brownian Motion of Nanoparticles
Three-dimensional in Situ Temperature Measurement in Microsystems Using Brownian Motion of Nanoparticles
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Author(s)
Chung, Kwanghun
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Abstract
Recent developments in microsystems for chemical and biological analysis offer significant advantages over
conventional methods, such as precise manipulation of samples and control of microenvironment. For many
applications, the ability to control and measure temperature inside microfluidic devices is critical since temperature
often affects biological or chemical processes. To address this need, we developed an in situ method for three-dimensionally
resolved temperature measurement in microsystems. The temperature of the surrounding fluid is
correlated from Brownian diffusion of suspended nanoparticles. We use video-microscopy in combination with image
analysis software to selectively track nanoparticles in the focal plane. This method is superior with regards to
reproducibility and reduced systematic errors since measuring Brownian diffusivity does not rely on fluorescence
intensity or lifetime of fluorophores. The efficacy of the method is demonstrated by measuring spatial temperature
profiles in various microfluidic devices that generate temperature gradients and by comparing these results with
numerical simulations. We show that the method is accurate and can be used to extract spatial temperature
variations in three dimensions. Compared to conventional methods that require expensive multiphoton optical
sectioning setups, this technique is simple and inexpensive. In addition, we demonstrate the capability of this method
as an in situ tool for simultaneously observing live cells under the microscope and monitoring the local temperature of
the cell medium without biochemical interference, which is crucial for quantitative studies of cells in microfluidic
devices.
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Date Issued
2009-10-14
Extent
31:06 minutes
Resource Type
Moving Image
Resource Subtype
Lecture