Microdissection and whole mount scanning electron microscopy visualization of mouse choroid plexus
- Author
- Elien Van Wonterghem (UGent) , Lien Van Hoecke (UGent) , Griet Van Imschoot (UGent) , Daan Verhaege (UGent) , Marlies Burgelman (UGent) and Roosmarijn Vandenbroucke (UGent)
- Organization
- Project
- Abstract
- The choroid plexus (CP), a highly vascularized structure protruding into the ventricles of the brain, is one of the most understudied tissues in neuroscience. As it is becoming increasingly clear that this tiny structure plays a crucial role in health and disease of the central nervous system (CNS), it is of utmost importance to properly dissect the CP out of the brain ventricles in a way that allows downstream processing, ranging from functional to structural analysis. Here, isolation of the lateral and fourth brain ventricle mouse CP without the need for specialized tools or equipment is described. This isolation technique preserves the viability, function, and structure of cells within the CP. On account of its high vascularization, the CP can be visualized floating inside the ventricular cavities of the brain using a binocular microscope. However, transcardial perfusion required for downstream analysis can complicate the identification of the CP tissue. Depending on the further processing steps (e.g., RNA and protein analysis), this can be solved by visualizing the CP via transcardial perfusion with bromophenol blue. After isolation, the CP can be processed using several techniques, including RNA, protein, or single cell analysis, to gain further understanding on the function of this special brain structure. Here, scanning electron microscopy (SEM) on whole mount CP is used to get an overall view of the structure.
- Keywords
- CEREBROSPINAL FLUID INTERFACE, BARRIER, BRAIN, DISEASE, PHYSIOLOGY
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01GSZAGHCG1NCFA3XT30EBYJZ0
- MLA
- Van Wonterghem, Elien, et al. “Microdissection and Whole Mount Scanning Electron Microscopy Visualization of Mouse Choroid Plexus.” JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, no. 190, 2022, doi:10.3791/64733.
- APA
- Van Wonterghem, E., Van Hoecke, L., Van Imschoot, G., Verhaege, D., Burgelman, M., & Vandenbroucke, R. (2022). Microdissection and whole mount scanning electron microscopy visualization of mouse choroid plexus. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, (190). https://doi.org/10.3791/64733
- Chicago author-date
- Van Wonterghem, Elien, Lien Van Hoecke, Griet Van Imschoot, Daan Verhaege, Marlies Burgelman, and Roosmarijn Vandenbroucke. 2022. “Microdissection and Whole Mount Scanning Electron Microscopy Visualization of Mouse Choroid Plexus.” JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, no. 190. https://doi.org/10.3791/64733.
- Chicago author-date (all authors)
- Van Wonterghem, Elien, Lien Van Hoecke, Griet Van Imschoot, Daan Verhaege, Marlies Burgelman, and Roosmarijn Vandenbroucke. 2022. “Microdissection and Whole Mount Scanning Electron Microscopy Visualization of Mouse Choroid Plexus.” JOVE-JOURNAL OF VISUALIZED EXPERIMENTS (190). doi:10.3791/64733.
- Vancouver
- 1.Van Wonterghem E, Van Hoecke L, Van Imschoot G, Verhaege D, Burgelman M, Vandenbroucke R. Microdissection and whole mount scanning electron microscopy visualization of mouse choroid plexus. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS. 2022;(190).
- IEEE
- [1]E. Van Wonterghem, L. Van Hoecke, G. Van Imschoot, D. Verhaege, M. Burgelman, and R. Vandenbroucke, “Microdissection and whole mount scanning electron microscopy visualization of mouse choroid plexus,” JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, no. 190, 2022.
@article{01GSZAGHCG1NCFA3XT30EBYJZ0,
abstract = {{The choroid plexus (CP), a highly vascularized structure protruding into the ventricles of the brain, is one of the most understudied tissues in neuroscience. As it is becoming increasingly clear that this tiny structure plays a crucial role in health and disease of the central nervous system (CNS), it is of utmost importance to properly dissect the CP out of the brain ventricles in a way that allows downstream processing, ranging from functional to structural analysis. Here, isolation of the lateral and fourth brain ventricle mouse CP without the need for specialized tools or equipment is described. This isolation technique preserves the viability, function, and structure of cells within the CP. On account of its high vascularization, the CP can be visualized floating inside the ventricular cavities of the brain using a binocular microscope. However, transcardial perfusion required for downstream analysis can complicate the identification of the CP tissue. Depending on the further processing steps (e.g., RNA and protein analysis), this can be solved by visualizing the CP via transcardial perfusion with bromophenol blue. After isolation, the CP can be processed using several techniques, including RNA, protein, or single cell analysis, to gain further understanding on the function of this special brain structure. Here, scanning electron microscopy (SEM) on whole mount CP is used to get an overall view of the structure.}},
articleno = {{e64733}},
author = {{Van Wonterghem, Elien and Van Hoecke, Lien and Van Imschoot, Griet and Verhaege, Daan and Burgelman, Marlies and Vandenbroucke, Roosmarijn}},
issn = {{1940-087X}},
journal = {{JOVE-JOURNAL OF VISUALIZED EXPERIMENTS}},
keywords = {{CEREBROSPINAL FLUID INTERFACE,BARRIER,BRAIN,DISEASE,PHYSIOLOGY}},
language = {{eng}},
number = {{190}},
pages = {{13}},
title = {{Microdissection and whole mount scanning electron microscopy visualization of mouse choroid plexus}},
url = {{http://doi.org/10.3791/64733}},
year = {{2022}},
}
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