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Yersinia enterocolitica detection in pork products : evaluation of isolation protocols

Maria Francesca Peruzy, M. Aponte, Y. T. R. Proroga, F. Capuano, D. Cristiano, E. Delibato, Kurt Houf (UGent) and N. Murru
(2020) FOOD MICROBIOLOGY. 92.
Author
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Abstract
Conventional methods for Yersinia enterocolitica detection in food samples are generally considered inadequate. Problems arise from the presence of the so-called "background flora", coupled to the low contamination level of the pathogen. Since, data on the microbial ecology occurring in competitive microflora are still lacking, MALDI TOF MS was used for strains 'identification after enrichment in PSB or ITC broths, and after plating on selective CIN medium at different incubation times. SYBR Green Real time PCR was used for the Y. enterocolitica strains' detection (4/O:3, 1A/O:5) in experimentally contaminated foods, as well as in naturally contaminated samples. A higher number of different bacterial genera (10 on CIN and 18 on PCA) was recorded after enrichment in PSB, whilst enrichment in ITC led to recovery of 6 and 10 genera on CIN and PCA, respectively. Yersiniaceae was the dominant family on the first day of incubation, but on the second day the percentage of isolation considerably decreased. By testing experimentally contaminated samples, substantial difficulties were encountered. The biotype 1A was always detected, whereas strain 4/0:3 proved to be poorly competitive. Based on the data, the enrichment media PSB and ITC, currently proposed for Y. enterocolitica detection, need to be improved to promote a successful pathogen's recovery.
Keywords
Yersinia enterocolitica 4/O:3, Y. enterocolitica 1A/O:5, "Background, flora", MALDI-TOF MS, SYBR Green real time PCR, IDENTIFICATION, CARCASSES, GENES, TIME

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MLA
Peruzy, Maria Francesca, et al. “Yersinia Enterocolitica Detection in Pork Products : Evaluation of Isolation Protocols.” FOOD MICROBIOLOGY, vol. 92, Academic Press LTD - Elsevier Science LTD, 2020, doi:10.1016/j.fm.2020.103593.
APA
Peruzy, M. F., Aponte, M., Proroga, Y. T. R., Capuano, F., Cristiano, D., Delibato, E., … Murru, N. (2020). Yersinia enterocolitica detection in pork products : evaluation of isolation protocols. FOOD MICROBIOLOGY, 92. https://doi.org/10.1016/j.fm.2020.103593
Chicago author-date
Peruzy, Maria Francesca, M. Aponte, Y. T. R. Proroga, F. Capuano, D. Cristiano, E. Delibato, Kurt Houf, and N. Murru. 2020. “Yersinia Enterocolitica Detection in Pork Products : Evaluation of Isolation Protocols.” FOOD MICROBIOLOGY 92. https://doi.org/10.1016/j.fm.2020.103593.
Chicago author-date (all authors)
Peruzy, Maria Francesca, M. Aponte, Y. T. R. Proroga, F. Capuano, D. Cristiano, E. Delibato, Kurt Houf, and N. Murru. 2020. “Yersinia Enterocolitica Detection in Pork Products : Evaluation of Isolation Protocols.” FOOD MICROBIOLOGY 92. doi:10.1016/j.fm.2020.103593.
Vancouver
1.
Peruzy MF, Aponte M, Proroga YTR, Capuano F, Cristiano D, Delibato E, et al. Yersinia enterocolitica detection in pork products : evaluation of isolation protocols. FOOD MICROBIOLOGY. 2020;92.
IEEE
[1]
M. F. Peruzy et al., “Yersinia enterocolitica detection in pork products : evaluation of isolation protocols,” FOOD MICROBIOLOGY, vol. 92, 2020.
@article{01GV2Z5V0NY4EJBPBY8DS0VKGF,
  abstract     = {{Conventional methods for Yersinia enterocolitica detection in food samples are generally considered inadequate. Problems arise from the presence of the so-called "background flora", coupled to the low contamination level of the pathogen. Since, data on the microbial ecology occurring in competitive microflora are still lacking, MALDI TOF MS was used for strains 'identification after enrichment in PSB or ITC broths, and after plating on selective CIN medium at different incubation times. SYBR Green Real time PCR was used for the Y. enterocolitica strains' detection (4/O:3, 1A/O:5) in experimentally contaminated foods, as well as in naturally contaminated samples. A higher number of different bacterial genera (10 on CIN and 18 on PCA) was recorded after enrichment in PSB, whilst enrichment in ITC led to recovery of 6 and 10 genera on CIN and PCA, respectively. Yersiniaceae was the dominant family on the first day of incubation, but on the second day the percentage of isolation considerably decreased. By testing experimentally contaminated samples, substantial difficulties were encountered. The biotype 1A was always detected, whereas strain 4/0:3 proved to be poorly competitive. Based on the data, the enrichment media PSB and ITC, currently proposed for Y. enterocolitica detection, need to be improved to promote a successful pathogen's recovery.}},
  articleno    = {{103593}},
  author       = {{Peruzy, Maria Francesca and  Aponte, M. and  Proroga, Y. T. R. and  Capuano, F. and  Cristiano, D. and  Delibato, E. and Houf, Kurt and  Murru, N.}},
  issn         = {{0740-0020}},
  journal      = {{FOOD MICROBIOLOGY}},
  keywords     = {{Yersinia enterocolitica 4/O:3,Y. enterocolitica 1A/O:5,"Background,flora",MALDI-TOF MS,SYBR Green real time PCR,IDENTIFICATION,CARCASSES,GENES,TIME}},
  language     = {{eng}},
  pages        = {{8}},
  publisher    = {{Academic Press LTD - Elsevier Science LTD}},
  title        = {{Yersinia enterocolitica detection in pork products : evaluation of isolation protocols}},
  url          = {{http://doi.org/10.1016/j.fm.2020.103593}},
  volume       = {{92}},
  year         = {{2020}},
}
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