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Improved sample preparation for CE-LIF analysis of plant N-glycans

Bieke Nagels (UGent) , Francis Santens (UGent) , Koen Weterings, Els Van Damme (UGent) and Nico Callewaert (UGent)
(2011) ELECTROPHORESIS. 32(24). p.3482-3490
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Abstract
In view of glycomics studies in plants, it is important to have sensitive tools that allow one to analyze and characterize the N-glycans present on plant proteins in different species. Earlier methods combined plant-based sample preparations with CE-LIF N-glycan analysis but suffered from background contaminations, often resulting in non-reproducible results. This publication describes a reproducible and sensitive protocol for the preparation and analysis of plant N-glycans, based on a combination of the in-gel release method and N-glycan analysis on a multicapillary DNA sequencer. Our protocol makes it possible to analyze plant N-glycans starting from low amounts of plant material with highly reproducible results. The developed protocol was validated for different plant species and plant cells.
Keywords
ANTIBODIES, EXPRESSION, CELLS, PROTEINS, GLYCOPROTEINS, GLYCOSYLATION, PROCESSING ENZYMES, NICOTIANA-BENTHAMIANA, Arabidopsis thaliana, CE-LIF, In-gel release, SDS-PAGE, N-glycan analysis, GENERATION, GROWTH

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MLA
Nagels, Bieke, et al. “Improved Sample Preparation for CE-LIF Analysis of Plant N-Glycans.” ELECTROPHORESIS, vol. 32, no. 24, 2011, pp. 3482–90, doi:10.1002/elps.201100268.
APA
Nagels, B., Santens, F., Weterings, K., Van Damme, E., & Callewaert, N. (2011). Improved sample preparation for CE-LIF analysis of plant N-glycans. ELECTROPHORESIS, 32(24), 3482–3490. https://doi.org/10.1002/elps.201100268
Chicago author-date
Nagels, Bieke, Francis Santens, Koen Weterings, Els Van Damme, and Nico Callewaert. 2011. “Improved Sample Preparation for CE-LIF Analysis of Plant N-Glycans.” ELECTROPHORESIS 32 (24): 3482–90. https://doi.org/10.1002/elps.201100268.
Chicago author-date (all authors)
Nagels, Bieke, Francis Santens, Koen Weterings, Els Van Damme, and Nico Callewaert. 2011. “Improved Sample Preparation for CE-LIF Analysis of Plant N-Glycans.” ELECTROPHORESIS 32 (24): 3482–3490. doi:10.1002/elps.201100268.
Vancouver
1.
Nagels B, Santens F, Weterings K, Van Damme E, Callewaert N. Improved sample preparation for CE-LIF analysis of plant N-glycans. ELECTROPHORESIS. 2011;32(24):3482–90.
IEEE
[1]
B. Nagels, F. Santens, K. Weterings, E. Van Damme, and N. Callewaert, “Improved sample preparation for CE-LIF analysis of plant N-glycans,” ELECTROPHORESIS, vol. 32, no. 24, pp. 3482–3490, 2011.
@article{2034567,
  abstract     = {{In view of glycomics studies in plants, it is important to have sensitive tools that allow one to analyze and characterize the N-glycans present on plant proteins in different species. Earlier methods combined plant-based sample preparations with CE-LIF N-glycan analysis but suffered from background contaminations, often resulting in non-reproducible results. This publication describes a reproducible and sensitive protocol for the preparation and analysis of plant N-glycans, based on a combination of the in-gel release method and N-glycan analysis on a multicapillary DNA sequencer. Our protocol makes it possible to analyze plant N-glycans starting from low amounts of plant material with highly reproducible results. The developed protocol was validated for different plant species and plant cells.}},
  author       = {{Nagels, Bieke and Santens, Francis and Weterings, Koen and Van Damme, Els and Callewaert, Nico}},
  issn         = {{0173-0835}},
  journal      = {{ELECTROPHORESIS}},
  keywords     = {{ANTIBODIES,EXPRESSION,CELLS,PROTEINS,GLYCOPROTEINS,GLYCOSYLATION,PROCESSING ENZYMES,NICOTIANA-BENTHAMIANA,Arabidopsis thaliana,CE-LIF,In-gel release,SDS-PAGE,N-glycan analysis,GENERATION,GROWTH}},
  language     = {{eng}},
  number       = {{24}},
  pages        = {{3482--3490}},
  title        = {{Improved sample preparation for CE-LIF analysis of plant N-glycans}},
  url          = {{http://doi.org/10.1002/elps.201100268}},
  volume       = {{32}},
  year         = {{2011}},
}

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