Improved sample preparation for CE-LIF analysis of plant N-glycans
- Author
- Bieke Nagels (UGent) , Francis Santens (UGent) , Koen Weterings, Els Van Damme (UGent) and Nico Callewaert (UGent)
- Organization
- Abstract
- In view of glycomics studies in plants, it is important to have sensitive tools that allow one to analyze and characterize the N-glycans present on plant proteins in different species. Earlier methods combined plant-based sample preparations with CE-LIF N-glycan analysis but suffered from background contaminations, often resulting in non-reproducible results. This publication describes a reproducible and sensitive protocol for the preparation and analysis of plant N-glycans, based on a combination of the in-gel release method and N-glycan analysis on a multicapillary DNA sequencer. Our protocol makes it possible to analyze plant N-glycans starting from low amounts of plant material with highly reproducible results. The developed protocol was validated for different plant species and plant cells.
- Keywords
- ANTIBODIES, EXPRESSION, CELLS, PROTEINS, GLYCOPROTEINS, GLYCOSYLATION, PROCESSING ENZYMES, NICOTIANA-BENTHAMIANA, Arabidopsis thaliana, CE-LIF, In-gel release, SDS-PAGE, N-glycan analysis, GENERATION, GROWTH
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-2034567
- MLA
- Nagels, Bieke, et al. “Improved Sample Preparation for CE-LIF Analysis of Plant N-Glycans.” ELECTROPHORESIS, vol. 32, no. 24, 2011, pp. 3482–90, doi:10.1002/elps.201100268.
- APA
- Nagels, B., Santens, F., Weterings, K., Van Damme, E., & Callewaert, N. (2011). Improved sample preparation for CE-LIF analysis of plant N-glycans. ELECTROPHORESIS, 32(24), 3482–3490. https://doi.org/10.1002/elps.201100268
- Chicago author-date
- Nagels, Bieke, Francis Santens, Koen Weterings, Els Van Damme, and Nico Callewaert. 2011. “Improved Sample Preparation for CE-LIF Analysis of Plant N-Glycans.” ELECTROPHORESIS 32 (24): 3482–90. https://doi.org/10.1002/elps.201100268.
- Chicago author-date (all authors)
- Nagels, Bieke, Francis Santens, Koen Weterings, Els Van Damme, and Nico Callewaert. 2011. “Improved Sample Preparation for CE-LIF Analysis of Plant N-Glycans.” ELECTROPHORESIS 32 (24): 3482–3490. doi:10.1002/elps.201100268.
- Vancouver
- 1.Nagels B, Santens F, Weterings K, Van Damme E, Callewaert N. Improved sample preparation for CE-LIF analysis of plant N-glycans. ELECTROPHORESIS. 2011;32(24):3482–90.
- IEEE
- [1]B. Nagels, F. Santens, K. Weterings, E. Van Damme, and N. Callewaert, “Improved sample preparation for CE-LIF analysis of plant N-glycans,” ELECTROPHORESIS, vol. 32, no. 24, pp. 3482–3490, 2011.
@article{2034567, abstract = {{In view of glycomics studies in plants, it is important to have sensitive tools that allow one to analyze and characterize the N-glycans present on plant proteins in different species. Earlier methods combined plant-based sample preparations with CE-LIF N-glycan analysis but suffered from background contaminations, often resulting in non-reproducible results. This publication describes a reproducible and sensitive protocol for the preparation and analysis of plant N-glycans, based on a combination of the in-gel release method and N-glycan analysis on a multicapillary DNA sequencer. Our protocol makes it possible to analyze plant N-glycans starting from low amounts of plant material with highly reproducible results. The developed protocol was validated for different plant species and plant cells.}}, author = {{Nagels, Bieke and Santens, Francis and Weterings, Koen and Van Damme, Els and Callewaert, Nico}}, issn = {{0173-0835}}, journal = {{ELECTROPHORESIS}}, keywords = {{ANTIBODIES,EXPRESSION,CELLS,PROTEINS,GLYCOPROTEINS,GLYCOSYLATION,PROCESSING ENZYMES,NICOTIANA-BENTHAMIANA,Arabidopsis thaliana,CE-LIF,In-gel release,SDS-PAGE,N-glycan analysis,GENERATION,GROWTH}}, language = {{eng}}, number = {{24}}, pages = {{3482--3490}}, title = {{Improved sample preparation for CE-LIF analysis of plant N-glycans}}, url = {{http://doi.org/10.1002/elps.201100268}}, volume = {{32}}, year = {{2011}}, }
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