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The skin microbiome of caspase-14-deficient mice shows mild dysbiosis

Malgorzata Kubica (UGent) , Falk Hildebrand, Brigitta Brinkman (UGent) , Dirk Goossens, Jurgen Del Favero, Ken Vercammen, Pierre Cornelis, Jens-Michael Schröder, Peter Vandenabeele (UGent) , Jeroen Raes, et al.
(2014) EXPERIMENTAL DERMATOLOGY. 23(8). p.561-567
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Abstract
Caspase-14, an important proteinase involved in filaggrin catabolism, is mainly active in terminally differentiating keratinocytes, where it is required for the generation of skin natural moisturizing factors (NMFs). Consequently, caspase-14 deficient epidermis is characterized by reduced levels of NMFs such as urocanic acid and 2-pyrrolidone-5-carboxylic acid. Patients suffering from filaggrin deficiency are prone to develop atopic dermatitis, which is accompanied with increased microbial burden. Among several reasons, this effect could be due to a decrease in filaggrin breakdown products. In this study, we found that caspase-14(-/-) mice show enhanced antibacterial response compared to wild-type mice when challenged with bacteria. Therefore, we compared the microbial communities between wild-type and caspase-14(-/-) mice by sequencing of bacterial 16S ribosomal RNA genes. We observed that caspase-14 ablation leads to an increase in bacterial richness and diversity during steadystate conditions. Although both wild-type and caspase-14(-/-) skin were dominated by the Firmicutes phylum, the Staphylococcaceae family was reduced in caspase-14(-/-) mice. Altogether, our data demonstrated that caspase-14 deficiency causes the imbalance of the skin-resident bacterial communities.
Keywords
DIVERSITY, INFLAMMATION, FILAGGRIN, DEFENSE, KERATINOCYTES, STRATUM-CORNEUM, BARRIER FUNCTION, COMMUNITY STRUCTURE, microbiome, caspase-14, antimicrobial activity, HAIRLESS MICE, DISEASE

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MLA
Kubica, Malgorzata, et al. “The Skin Microbiome of Caspase-14-Deficient Mice Shows Mild Dysbiosis.” EXPERIMENTAL DERMATOLOGY, vol. 23, no. 8, 2014, pp. 561–67, doi:10.1111/exd.12458.
APA
Kubica, M., Hildebrand, F., Brinkman, B., Goossens, D., Del Favero, J., Vercammen, K., … Declercq, W. (2014). The skin microbiome of caspase-14-deficient mice shows mild dysbiosis. EXPERIMENTAL DERMATOLOGY, 23(8), 561–567. https://doi.org/10.1111/exd.12458
Chicago author-date
Kubica, Malgorzata, Falk Hildebrand, Brigitta Brinkman, Dirk Goossens, Jurgen Del Favero, Ken Vercammen, Pierre Cornelis, et al. 2014. “The Skin Microbiome of Caspase-14-Deficient Mice Shows Mild Dysbiosis.” EXPERIMENTAL DERMATOLOGY 23 (8): 561–67. https://doi.org/10.1111/exd.12458.
Chicago author-date (all authors)
Kubica, Malgorzata, Falk Hildebrand, Brigitta Brinkman, Dirk Goossens, Jurgen Del Favero, Ken Vercammen, Pierre Cornelis, Jens-Michael Schröder, Peter Vandenabeele, Jeroen Raes, and Wim Declercq. 2014. “The Skin Microbiome of Caspase-14-Deficient Mice Shows Mild Dysbiosis.” EXPERIMENTAL DERMATOLOGY 23 (8): 561–567. doi:10.1111/exd.12458.
Vancouver
1.
Kubica M, Hildebrand F, Brinkman B, Goossens D, Del Favero J, Vercammen K, et al. The skin microbiome of caspase-14-deficient mice shows mild dysbiosis. EXPERIMENTAL DERMATOLOGY. 2014;23(8):561–7.
IEEE
[1]
M. Kubica et al., “The skin microbiome of caspase-14-deficient mice shows mild dysbiosis,” EXPERIMENTAL DERMATOLOGY, vol. 23, no. 8, pp. 561–567, 2014.
@article{5704312,
  abstract     = {{Caspase-14, an important proteinase involved in filaggrin catabolism, is mainly active in terminally differentiating keratinocytes, where it is required for the generation of skin natural moisturizing factors (NMFs). Consequently, caspase-14 deficient epidermis is characterized by reduced levels of NMFs such as urocanic acid and 2-pyrrolidone-5-carboxylic acid. Patients suffering from filaggrin deficiency are prone to develop atopic dermatitis, which is accompanied with increased microbial burden. Among several reasons, this effect could be due to a decrease in filaggrin breakdown products. In this study, we found that caspase-14(-/-) mice show enhanced antibacterial response compared to wild-type mice when challenged with bacteria. Therefore, we compared the microbial communities between wild-type and caspase-14(-/-) mice by sequencing of bacterial 16S ribosomal RNA genes. We observed that caspase-14 ablation leads to an increase in bacterial richness and diversity during steadystate conditions. Although both wild-type and caspase-14(-/-) skin were dominated by the Firmicutes phylum, the Staphylococcaceae family was reduced in caspase-14(-/-) mice. Altogether, our data demonstrated that caspase-14 deficiency causes the imbalance of the skin-resident bacterial communities.}},
  author       = {{Kubica, Malgorzata and Hildebrand, Falk and Brinkman, Brigitta and Goossens, Dirk and Del Favero, Jurgen and Vercammen, Ken and Cornelis, Pierre and Schröder, Jens-Michael and Vandenabeele, Peter and Raes, Jeroen and Declercq, Wim}},
  issn         = {{0906-6705}},
  journal      = {{EXPERIMENTAL DERMATOLOGY}},
  keywords     = {{DIVERSITY,INFLAMMATION,FILAGGRIN,DEFENSE,KERATINOCYTES,STRATUM-CORNEUM,BARRIER FUNCTION,COMMUNITY STRUCTURE,microbiome,caspase-14,antimicrobial activity,HAIRLESS MICE,DISEASE}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{561--567}},
  title        = {{The skin microbiome of caspase-14-deficient mice shows mild dysbiosis}},
  url          = {{http://doi.org/10.1111/exd.12458}},
  volume       = {{23}},
  year         = {{2014}},
}
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