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Occurrence and molecular characterization of Giardia duodenalis and Cryptosporidium spp. in sheep and goats reared under dairy husbandry systems in Greece

(2014) PARASITE. 21.
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Abstract
Giardia duodenalis and Cryptosporidium spp. are gastro-intestinal protozoa known to infect small ruminants. Both protozoa are also considered as a potential public health concern. The objective of this study was to determine their prevalence in lambs and goat kids kept under common Mediterranean dairy husbandry systems and to identify the species and genotypes infecting these small ruminants. In total, 684 faecal samples (429 from lambs and 255 from goat kids) were collected on 21 farms in Greece and examined using a quantitative immunofluorescence assay. G. duodenalis was detected in 37.3% of the lambs and 40.4% of the goat kids. On all but one of the farms G. duodenalis was detected. Most samples were typed as a mono-infection with G. duodenalis assemblage E, both on the beta-giardin gene and the triose phosphate isomerase gene. Only 10% of samples were typed as mixed assemblage A and E infections. The prevalence of Cryptosporidium spp. was 5.1% in lambs and 7.1% in goat kids. In total, 8 out of the 14 farms with a sheep flock and 7 out of the 14 farms with a goat flock were positive. Cryptosporidium parvum (subtype IId), C. ubiquitum and C. xiaoi were identified, the latter especially in goat kids. In conclusion, the results of the present study illustrate that G. duodenalis and Cryptosporidium spp. occur frequently on both sheep and goats farms. The prevalence of zoonotic genotypes or species was low, indicating a limited but existing risk for zoonotic infections.
Keywords
ENVIRONMENTAL CONTAMINATION, ZOONOTIC TRANSMISSION, LAMBS, PREVALENCE, HUMANS, SPAIN, KIDS, GENOTYPES, ASSEMBLAGE, ANIMALS, Genotyping, Prevalence, Dairy, Sheep, Goat, Cryptosporidium, Giardia

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MLA
Tzanidakis, Nikolaos, et al. “Occurrence and Molecular Characterization of Giardia Duodenalis and Cryptosporidium Spp. in Sheep and Goats Reared under Dairy Husbandry Systems in Greece.” PARASITE, vol. 21, 2014, doi:10.1051/parasite/2014048.
APA
Tzanidakis, N., Sotiraki, S., Claerebout, E., Ehsan, Md. A., Voutzourakis, N., Kostopoulou, D., … Geurden, T. (2014). Occurrence and molecular characterization of Giardia duodenalis and Cryptosporidium spp. in sheep and goats reared under dairy husbandry systems in Greece. PARASITE, 21. https://doi.org/10.1051/parasite/2014048
Chicago author-date
Tzanidakis, Nikolaos, Smaragda Sotiraki, Edwin Claerebout, Md. Amimul Ehsan, Nikolaos Voutzourakis, Despoina Kostopoulou, Stijn Casaert, Jozef Vercruysse, and Thomas Geurden. 2014. “Occurrence and Molecular Characterization of Giardia Duodenalis and Cryptosporidium Spp. in Sheep and Goats Reared under Dairy Husbandry Systems in Greece.” PARASITE 21. https://doi.org/10.1051/parasite/2014048.
Chicago author-date (all authors)
Tzanidakis, Nikolaos, Smaragda Sotiraki, Edwin Claerebout, Md. Amimul Ehsan, Nikolaos Voutzourakis, Despoina Kostopoulou, Stijn Casaert, Jozef Vercruysse, and Thomas Geurden. 2014. “Occurrence and Molecular Characterization of Giardia Duodenalis and Cryptosporidium Spp. in Sheep and Goats Reared under Dairy Husbandry Systems in Greece.” PARASITE 21. doi:10.1051/parasite/2014048.
Vancouver
1.
Tzanidakis N, Sotiraki S, Claerebout E, Ehsan MdA, Voutzourakis N, Kostopoulou D, et al. Occurrence and molecular characterization of Giardia duodenalis and Cryptosporidium spp. in sheep and goats reared under dairy husbandry systems in Greece. PARASITE. 2014;21.
IEEE
[1]
N. Tzanidakis et al., “Occurrence and molecular characterization of Giardia duodenalis and Cryptosporidium spp. in sheep and goats reared under dairy husbandry systems in Greece,” PARASITE, vol. 21, 2014.
@article{5719690,
  abstract     = {{Giardia duodenalis and Cryptosporidium spp. are gastro-intestinal protozoa known to infect small ruminants. Both protozoa are also considered as a potential public health concern. The objective of this study was to determine their prevalence in lambs and goat kids kept under common Mediterranean dairy husbandry systems and to identify the species and genotypes infecting these small ruminants. In total, 684 faecal samples (429 from lambs and 255 from goat kids) were collected on 21 farms in Greece and examined using a quantitative immunofluorescence assay. G. duodenalis was detected in 37.3% of the lambs and 40.4% of the goat kids. On all but one of the farms G. duodenalis was detected. Most samples were typed as a mono-infection with G. duodenalis assemblage E, both on the beta-giardin gene and the triose phosphate isomerase gene. Only 10% of samples were typed as mixed assemblage A and E infections. The prevalence of Cryptosporidium spp. was 5.1% in lambs and 7.1% in goat kids. In total, 8 out of the 14 farms with a sheep flock and 7 out of the 14 farms with a goat flock were positive. Cryptosporidium parvum (subtype IId), C. ubiquitum and C. xiaoi were identified, the latter especially in goat kids. In conclusion, the results of the present study illustrate that G. duodenalis and Cryptosporidium spp. occur frequently on both sheep and goats farms. The prevalence of zoonotic genotypes or species was low, indicating a limited but existing risk for zoonotic infections.}},
  articleno    = {{45}},
  author       = {{Tzanidakis, Nikolaos and Sotiraki, Smaragda and Claerebout, Edwin and Ehsan, Md. Amimul and Voutzourakis, Nikolaos and Kostopoulou, Despoina and Casaert, Stijn and Vercruysse, Jozef and Geurden, Thomas}},
  issn         = {{1252-607X}},
  journal      = {{PARASITE}},
  keywords     = {{ENVIRONMENTAL CONTAMINATION,ZOONOTIC TRANSMISSION,LAMBS,PREVALENCE,HUMANS,SPAIN,KIDS,GENOTYPES,ASSEMBLAGE,ANIMALS,Genotyping,Prevalence,Dairy,Sheep,Goat,Cryptosporidium,Giardia}},
  language     = {{eng}},
  pages        = {{7}},
  title        = {{Occurrence and molecular characterization of Giardia duodenalis and Cryptosporidium spp. in sheep and goats reared under dairy husbandry systems in Greece}},
  url          = {{http://doi.org/10.1051/parasite/2014048}},
  volume       = {{21}},
  year         = {{2014}},
}

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