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A novel probe for chemiluminescent image detection of proteins in two-dimensional gel electrophoresis

Jia Liu, Jin Ouyang, Willy Baeyens (UGent) , Joris Delanghe (UGent) , Zhenzhen Wang, Jinxiu Liu and Huiying Zhang
(2008) ELECTROPHORESIS. 29(3). p.716-725
Author
Organization
Abstract
Carrier ampholytes were found to enhance the chemiluminescence (CL) emission from the 3-aminophthalic hydrazide (luminol)-hydrogen peroxide system. They can be used as a chemiluminescent probe for rapid detection of major proteins in gels. This probe attracted much interest due to its ability to attach proteins, and to the possibility to combine it with separation techniques generating the CL emission directly. increased signal intensity was achieved employing optimized concentrations of the carrier ampholyte enhancer. The binding of carrier ampholyte to proteins was found to occur at the pI of the proteins. Proteins from different regions of the gels were identified by their matrix-assisted TOF mass spectra and by appropriate database search, the results illustrating the possibility of major protein detection in human serum. Direct CL image detection with the carrier ampholyte, probe can be applied for the detection of characteristic proteins in patients, i.e., proteins which cannot be detected without the probe.
Keywords
CAPILLARY-ELECTROPHORESIS, TELOMERASE ACTIVITY, SERUM COMPLEMENT LEVELS, nondenaturing 2-D gel electrophoresis, CHRONIC LYMPHOCYTIC-LEUKEMIA, human serum protein, carrier ampholyte, direct chemiluminescent imaging, PROTEOME, HEMOGLOBIN, COMPLEXES, PEPTIDES, IMMUNITY, SYSTEM

Citation

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MLA
Liu, Jia, et al. “A Novel Probe for Chemiluminescent Image Detection of Proteins in Two-Dimensional Gel Electrophoresis.” ELECTROPHORESIS, vol. 29, no. 3, WILEY-V C H VERLAG GMBH, 2008, pp. 716–25, doi:10.1002/elps.200700424.
APA
Liu, J., Ouyang, J., Baeyens, W., Delanghe, J., Wang, Z., Liu, J., & Zhang, H. (2008). A novel probe for chemiluminescent image detection of proteins in two-dimensional gel electrophoresis. ELECTROPHORESIS, 29(3), 716–725. https://doi.org/10.1002/elps.200700424
Chicago author-date
Liu, Jia, Jin Ouyang, Willy Baeyens, Joris Delanghe, Zhenzhen Wang, Jinxiu Liu, and Huiying Zhang. 2008. “A Novel Probe for Chemiluminescent Image Detection of Proteins in Two-Dimensional Gel Electrophoresis.” ELECTROPHORESIS 29 (3): 716–25. https://doi.org/10.1002/elps.200700424.
Chicago author-date (all authors)
Liu, Jia, Jin Ouyang, Willy Baeyens, Joris Delanghe, Zhenzhen Wang, Jinxiu Liu, and Huiying Zhang. 2008. “A Novel Probe for Chemiluminescent Image Detection of Proteins in Two-Dimensional Gel Electrophoresis.” ELECTROPHORESIS 29 (3): 716–725. doi:10.1002/elps.200700424.
Vancouver
1.
Liu J, Ouyang J, Baeyens W, Delanghe J, Wang Z, Liu J, et al. A novel probe for chemiluminescent image detection of proteins in two-dimensional gel electrophoresis. ELECTROPHORESIS. 2008;29(3):716–25.
IEEE
[1]
J. Liu et al., “A novel probe for chemiluminescent image detection of proteins in two-dimensional gel electrophoresis,” ELECTROPHORESIS, vol. 29, no. 3, pp. 716–725, 2008.
@article{599795,
  abstract     = {{Carrier ampholytes were found to enhance the chemiluminescence (CL) emission from the 3-aminophthalic hydrazide (luminol)-hydrogen peroxide system. They can be used as a chemiluminescent probe for rapid detection of major proteins in gels. This probe attracted much interest due to its ability to attach proteins, and to the possibility to combine it with separation techniques generating the CL emission directly. increased signal intensity was achieved employing optimized concentrations of the carrier ampholyte enhancer. The binding of carrier ampholyte to proteins was found to occur at the pI of the proteins. Proteins from different regions of the gels were identified by their matrix-assisted TOF mass spectra and by appropriate database search, the results illustrating the possibility of major protein detection in human serum. Direct CL image detection with the carrier ampholyte, probe can be applied for the detection of characteristic proteins in patients, i.e., proteins which cannot be detected without the probe.}},
  author       = {{Liu, Jia and Ouyang, Jin and Baeyens, Willy and Delanghe, Joris and Wang, Zhenzhen and Liu, Jinxiu and Zhang, Huiying}},
  issn         = {{0173-0835}},
  journal      = {{ELECTROPHORESIS}},
  keywords     = {{CAPILLARY-ELECTROPHORESIS,TELOMERASE ACTIVITY,SERUM COMPLEMENT LEVELS,nondenaturing 2-D gel electrophoresis,CHRONIC LYMPHOCYTIC-LEUKEMIA,human serum protein,carrier ampholyte,direct chemiluminescent imaging,PROTEOME,HEMOGLOBIN,COMPLEXES,PEPTIDES,IMMUNITY,SYSTEM}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{716--725}},
  publisher    = {{WILEY-V C H VERLAG GMBH}},
  title        = {{A novel probe for chemiluminescent image detection of proteins in two-dimensional gel electrophoresis}},
  url          = {{http://doi.org/10.1002/elps.200700424}},
  volume       = {{29}},
  year         = {{2008}},
}
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