- Author
- Henk Joos, Dirk Inzé (UGent) , Allan Caplan (UGent) , Monika Sormann, Marc Van Montagu (UGent) and Jeff Schell
- Organization
- Abstract
- Plant crown gall tumor cells result from the insertion and expression of a defined DNA sequence, called T-DNA, which is derived from the Ti plasmid, harbored by Agrobacterium tumefaciens strains. To study the function of the genes of the T-DNA of the nopaline Ti plasmid, pTiC58, a collection of mutants was isolated so that T-DNA genes are inactivated either separately or in various combinations. It was found that no single T-DNA gene or T-region border is absolutely essential for stable tumor formation. We have identified the gene responsible for synthesis in transformed cells of the phosphorylated sugar, agrocinopine, and at least three additional genes controlling the morphology of plant tumors. Two of these latter genes work together to inhibit shoot formation and ensure efficient tumorous growth. Inactivation of these genes can be suppressed by the addition of auxins. The third gene inhibits root formation and appears to play a role in the cytokinin-independent growth of transformed cells. Mutants missing all three genes do not induce tumors, nor shoot or root formation, although the mutant T-DNA sequence is transferred to plant cells.
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-6889730
- MLA
- Joos, Henk, et al. “Genetic Analysis of T-DNA Transcripts in Nopaline Crown Galls.” CELL, vol. 32, no. 4, 1983, pp. 1057–67, doi:10.1016/0092-8674(83)90290-8.
- APA
- Joos, H., Inzé, D., Caplan, A., Sormann, M., Van Montagu, M., & Schell, J. (1983). Genetic analysis of T-DNA transcripts in nopaline crown galls. CELL, 32(4), 1057–1067. https://doi.org/10.1016/0092-8674(83)90290-8
- Chicago author-date
- Joos, Henk, Dirk Inzé, Allan Caplan, Monika Sormann, Marc Van Montagu, and Jeff Schell. 1983. “Genetic Analysis of T-DNA Transcripts in Nopaline Crown Galls.” CELL 32 (4): 1057–67. https://doi.org/10.1016/0092-8674(83)90290-8.
- Chicago author-date (all authors)
- Joos, Henk, Dirk Inzé, Allan Caplan, Monika Sormann, Marc Van Montagu, and Jeff Schell. 1983. “Genetic Analysis of T-DNA Transcripts in Nopaline Crown Galls.” CELL 32 (4): 1057–1067. doi:10.1016/0092-8674(83)90290-8.
- Vancouver
- 1.Joos H, Inzé D, Caplan A, Sormann M, Van Montagu M, Schell J. Genetic analysis of T-DNA transcripts in nopaline crown galls. CELL. 1983;32(4):1057–67.
- IEEE
- [1]H. Joos, D. Inzé, A. Caplan, M. Sormann, M. Van Montagu, and J. Schell, “Genetic analysis of T-DNA transcripts in nopaline crown galls,” CELL, vol. 32, no. 4, pp. 1057–1067, 1983.
@article{6889730,
abstract = {{Plant crown gall tumor cells result from the insertion and expression of a defined DNA sequence, called T-DNA, which is derived from the Ti plasmid, harbored by Agrobacterium tumefaciens strains. To study the function of the genes of the T-DNA of the nopaline Ti plasmid, pTiC58, a collection of mutants was isolated so that T-DNA genes are inactivated either separately or in various combinations. It was found that no single T-DNA gene or T-region border is absolutely essential for stable tumor formation. We have identified the gene responsible for synthesis in transformed cells of the phosphorylated sugar, agrocinopine, and at least three additional genes controlling the morphology of plant tumors. Two of these latter genes work together to inhibit shoot formation and ensure efficient tumorous growth. Inactivation of these genes can be suppressed by the addition of auxins. The third gene inhibits root formation and appears to play a role in the cytokinin-independent growth of transformed cells. Mutants missing all three genes do not induce tumors, nor shoot or root formation, although the mutant T-DNA sequence is transferred to plant cells.}},
author = {{Joos, Henk and Inzé, Dirk and Caplan, Allan and Sormann, Monika and Van Montagu, Marc and Schell, Jeff}},
issn = {{0092-8674}},
journal = {{CELL}},
language = {{eng}},
number = {{4}},
pages = {{1057--1067}},
title = {{Genetic analysis of T-DNA transcripts in nopaline crown galls}},
url = {{http://doi.org/10.1016/0092-8674(83)90290-8}},
volume = {{32}},
year = {{1983}},
}
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