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Rapid alternative methods for detection of rifampicin resistance in Mycobacterium tuberculosis

Author
Organization
Abstract
Objective: To study the performance of three rapid low cost methods for the detection of rifampicin resistance. Methods: A panel of 20 coded Mycobacterium tuberculosis strains was tested blindly by the low cost methods: nitrate reductase, MTT and resazurin assays, and compared with the results obtained with the gold standard methods: the proportion method on Lowenstein-Jensen medium and the BACTEC TB 460 system. We have also tested two commercial tests: MGIT and INNO LiPA Rif.TB kit. Results: Complete agreement was observed among all methods. Conclusion: These three simple methods might become inexpensive alternative procedures for rapid detection of rifampicin resistance in low-resource countries.
Keywords
MTT, M. tuberculosis, resazurin, nitrate reductase assay, rifampicin, 3-(4_5-DIMETHYLTHIAZOL-2-YL)-2_5-DIPHENYL TETRAZOLIUM BROMIDE, MICROTITER ASSAY PLATE, DRUG SUSCEPTIBILITY, RADIOMETRIC METHOD, INEXPENSIVE METHOD, INDICATOR, SENSITIVITY, MTT

Citation

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MLA
Lemus, D., et al. “Rapid Alternative Methods for Detection of Rifampicin Resistance in Mycobacterium Tuberculosis.” JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, vol. 54, no. 1, 2004, pp. 130–33, doi:10.1093/jac/dkh320.
APA
Lemus, D., Martin, A., Montoro, E., Portaels, F., & Palomino, J. C. (2004). Rapid alternative methods for detection of rifampicin resistance in Mycobacterium tuberculosis. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 54(1), 130–133. https://doi.org/10.1093/jac/dkh320
Chicago author-date
Lemus, D, Anandi Martin, E Montoro, F Portaels, and Juan Carlos Palomino. 2004. “Rapid Alternative Methods for Detection of Rifampicin Resistance in Mycobacterium Tuberculosis.” JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY 54 (1): 130–33. https://doi.org/10.1093/jac/dkh320.
Chicago author-date (all authors)
Lemus, D, Anandi Martin, E Montoro, F Portaels, and Juan Carlos Palomino. 2004. “Rapid Alternative Methods for Detection of Rifampicin Resistance in Mycobacterium Tuberculosis.” JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY 54 (1): 130–133. doi:10.1093/jac/dkh320.
Vancouver
1.
Lemus D, Martin A, Montoro E, Portaels F, Palomino JC. Rapid alternative methods for detection of rifampicin resistance in Mycobacterium tuberculosis. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. 2004;54(1):130–3.
IEEE
[1]
D. Lemus, A. Martin, E. Montoro, F. Portaels, and J. C. Palomino, “Rapid alternative methods for detection of rifampicin resistance in Mycobacterium tuberculosis,” JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, vol. 54, no. 1, pp. 130–133, 2004.
@article{7187162,
  abstract     = {{Objective: To study the performance of three rapid low cost methods for the detection of rifampicin resistance. 
Methods: A panel of 20 coded Mycobacterium tuberculosis strains was tested blindly by the low cost methods: nitrate reductase, MTT and resazurin assays, and compared with the results obtained with the gold standard methods: the proportion method on Lowenstein-Jensen medium and the BACTEC TB 460 system. We have also tested two commercial tests: MGIT and INNO LiPA Rif.TB kit. 
Results: Complete agreement was observed among all methods. 
Conclusion: These three simple methods might become inexpensive alternative procedures for rapid detection of rifampicin resistance in low-resource countries.}},
  author       = {{Lemus, D and Martin, Anandi and Montoro, E and Portaels, F and Palomino, Juan Carlos}},
  issn         = {{0305-7453}},
  journal      = {{JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY}},
  keywords     = {{MTT,M. tuberculosis,resazurin,nitrate reductase assay,rifampicin,3-(4_5-DIMETHYLTHIAZOL-2-YL)-2_5-DIPHENYL TETRAZOLIUM BROMIDE,MICROTITER ASSAY PLATE,DRUG SUSCEPTIBILITY,RADIOMETRIC METHOD,INEXPENSIVE METHOD,INDICATOR,SENSITIVITY,MTT}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{130--133}},
  title        = {{Rapid alternative methods for detection of rifampicin resistance in Mycobacterium tuberculosis}},
  url          = {{http://doi.org/10.1093/jac/dkh320}},
  volume       = {{54}},
  year         = {{2004}},
}

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