Rapid alternative methods for detection of rifampicin resistance in Mycobacterium tuberculosis
- Author
- D Lemus, Anandi Martin (UGent) , E Montoro, F Portaels and Juan Carlos Palomino (UGent)
- Organization
- Abstract
- Objective: To study the performance of three rapid low cost methods for the detection of rifampicin resistance. Methods: A panel of 20 coded Mycobacterium tuberculosis strains was tested blindly by the low cost methods: nitrate reductase, MTT and resazurin assays, and compared with the results obtained with the gold standard methods: the proportion method on Lowenstein-Jensen medium and the BACTEC TB 460 system. We have also tested two commercial tests: MGIT and INNO LiPA Rif.TB kit. Results: Complete agreement was observed among all methods. Conclusion: These three simple methods might become inexpensive alternative procedures for rapid detection of rifampicin resistance in low-resource countries.
- Keywords
- MTT, M. tuberculosis, resazurin, nitrate reductase assay, rifampicin, 3-(4_5-DIMETHYLTHIAZOL-2-YL)-2_5-DIPHENYL TETRAZOLIUM BROMIDE, MICROTITER ASSAY PLATE, DRUG SUSCEPTIBILITY, RADIOMETRIC METHOD, INEXPENSIVE METHOD, INDICATOR, SENSITIVITY, MTT
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-7187162
- MLA
- Lemus, D., et al. “Rapid Alternative Methods for Detection of Rifampicin Resistance in Mycobacterium Tuberculosis.” JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, vol. 54, no. 1, 2004, pp. 130–33, doi:10.1093/jac/dkh320.
- APA
- Lemus, D., Martin, A., Montoro, E., Portaels, F., & Palomino, J. C. (2004). Rapid alternative methods for detection of rifampicin resistance in Mycobacterium tuberculosis. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 54(1), 130–133. https://doi.org/10.1093/jac/dkh320
- Chicago author-date
- Lemus, D, Anandi Martin, E Montoro, F Portaels, and Juan Carlos Palomino. 2004. “Rapid Alternative Methods for Detection of Rifampicin Resistance in Mycobacterium Tuberculosis.” JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY 54 (1): 130–33. https://doi.org/10.1093/jac/dkh320.
- Chicago author-date (all authors)
- Lemus, D, Anandi Martin, E Montoro, F Portaels, and Juan Carlos Palomino. 2004. “Rapid Alternative Methods for Detection of Rifampicin Resistance in Mycobacterium Tuberculosis.” JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY 54 (1): 130–133. doi:10.1093/jac/dkh320.
- Vancouver
- 1.Lemus D, Martin A, Montoro E, Portaels F, Palomino JC. Rapid alternative methods for detection of rifampicin resistance in Mycobacterium tuberculosis. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. 2004;54(1):130–3.
- IEEE
- [1]D. Lemus, A. Martin, E. Montoro, F. Portaels, and J. C. Palomino, “Rapid alternative methods for detection of rifampicin resistance in Mycobacterium tuberculosis,” JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, vol. 54, no. 1, pp. 130–133, 2004.
@article{7187162, abstract = {{Objective: To study the performance of three rapid low cost methods for the detection of rifampicin resistance. Methods: A panel of 20 coded Mycobacterium tuberculosis strains was tested blindly by the low cost methods: nitrate reductase, MTT and resazurin assays, and compared with the results obtained with the gold standard methods: the proportion method on Lowenstein-Jensen medium and the BACTEC TB 460 system. We have also tested two commercial tests: MGIT and INNO LiPA Rif.TB kit. Results: Complete agreement was observed among all methods. Conclusion: These three simple methods might become inexpensive alternative procedures for rapid detection of rifampicin resistance in low-resource countries.}}, author = {{Lemus, D and Martin, Anandi and Montoro, E and Portaels, F and Palomino, Juan Carlos}}, issn = {{0305-7453}}, journal = {{JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY}}, keywords = {{MTT,M. tuberculosis,resazurin,nitrate reductase assay,rifampicin,3-(4_5-DIMETHYLTHIAZOL-2-YL)-2_5-DIPHENYL TETRAZOLIUM BROMIDE,MICROTITER ASSAY PLATE,DRUG SUSCEPTIBILITY,RADIOMETRIC METHOD,INEXPENSIVE METHOD,INDICATOR,SENSITIVITY,MTT}}, language = {{eng}}, number = {{1}}, pages = {{130--133}}, title = {{Rapid alternative methods for detection of rifampicin resistance in Mycobacterium tuberculosis}}, url = {{http://doi.org/10.1093/jac/dkh320}}, volume = {{54}}, year = {{2004}}, }
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