- Author
- Patrice Petillot, Christophe Lahorte, Elena Bonanno, Alberto Signore, Steve Lancel, Philippe Marchetti, Benoit Vallet, Guido Slegers (UGent) and Remi Neviere
- Organization
- Abstract
- Acute inflammatory response to lipopolysaccharicle (LPS) exposure is typically associated with cardiac myocyte apoptosis, which is difficult to quantity because of heart tissue specificity. We report here that radioidinated Annexin V (I-123,I-125-AnxV), a specific ligand of phosphaticylserine exposed by apoptotic cells, allows tissue detection of apoptosis in LPS-treated rat hearts. Heart I-123-AnxV uptake was significantly increased in all cardiac territories of LPS-treated rats. In contrast, I-123-human serum albumin myocardial uptake was only slightly increased in LPS-treated rat hearts, suggesting limited changes in vascular protein permeability. Autoradiography of enclotoxin-treated rat heart sections with I-125-AnxV in association with deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling and caspase 3 staining allows identification of double positive cardiac myocytes. Inhibition of apoptosis by caspase inhibitors (i.e., ZVAD.fmk and DEVD.cmk) reduced I-123-AnxV myocardial uptake in LPS-treated rats. Eventually, endotoxin-treated rats displayed pathological uptake of Tc-99m-annexin in the cardiac mediastinal region whereas zVAD.fmk reduced Tc-99m-annexin mediastinal uptake. Our results show that radioactive I-123-AnxV signal emerging from LPS-treated rat hearts could be related to the activation of caspase-dependent apoptotic pathway in cardiac myocytes.
- Keywords
- HEART-FAILURE, caspase inhibitors, IN-VITRO, PHOSPHATIDYLSERINE, SEPSIS, RATS, INFLAMMATION, REPERFUSION, INHIBITION, lipopolysaccharide, myocardial apoptosis, annexin V, MYOCYTE APOPTOSIS, MYOCARDIAL DYSFUNCTION
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-742230
- MLA
- Petillot, Patrice, et al. “Annexin V Detection of Lipopolysaccharide-Induced Cardiac Apoptosis.” SHOCK, vol. 27, no. 1, 2007, pp. 69–74, doi:10.1097/01.shk.0000235085.56100.38.
- APA
- Petillot, P., Lahorte, C., Bonanno, E., Signore, A., Lancel, S., Marchetti, P., … Neviere, R. (2007). Annexin V detection of lipopolysaccharide-induced cardiac apoptosis. SHOCK, 27(1), 69–74. https://doi.org/10.1097/01.shk.0000235085.56100.38
- Chicago author-date
- Petillot, Patrice, Christophe Lahorte, Elena Bonanno, Alberto Signore, Steve Lancel, Philippe Marchetti, Benoit Vallet, Guido Slegers, and Remi Neviere. 2007. “Annexin V Detection of Lipopolysaccharide-Induced Cardiac Apoptosis.” SHOCK 27 (1): 69–74. https://doi.org/10.1097/01.shk.0000235085.56100.38.
- Chicago author-date (all authors)
- Petillot, Patrice, Christophe Lahorte, Elena Bonanno, Alberto Signore, Steve Lancel, Philippe Marchetti, Benoit Vallet, Guido Slegers, and Remi Neviere. 2007. “Annexin V Detection of Lipopolysaccharide-Induced Cardiac Apoptosis.” SHOCK 27 (1): 69–74. doi:10.1097/01.shk.0000235085.56100.38.
- Vancouver
- 1.Petillot P, Lahorte C, Bonanno E, Signore A, Lancel S, Marchetti P, et al. Annexin V detection of lipopolysaccharide-induced cardiac apoptosis. SHOCK. 2007;27(1):69–74.
- IEEE
- [1]P. Petillot et al., “Annexin V detection of lipopolysaccharide-induced cardiac apoptosis,” SHOCK, vol. 27, no. 1, pp. 69–74, 2007.
@article{742230, abstract = {{Acute inflammatory response to lipopolysaccharicle (LPS) exposure is typically associated with cardiac myocyte apoptosis, which is difficult to quantity because of heart tissue specificity. We report here that radioidinated Annexin V (I-123,I-125-AnxV), a specific ligand of phosphaticylserine exposed by apoptotic cells, allows tissue detection of apoptosis in LPS-treated rat hearts. Heart I-123-AnxV uptake was significantly increased in all cardiac territories of LPS-treated rats. In contrast, I-123-human serum albumin myocardial uptake was only slightly increased in LPS-treated rat hearts, suggesting limited changes in vascular protein permeability. Autoradiography of enclotoxin-treated rat heart sections with I-125-AnxV in association with deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling and caspase 3 staining allows identification of double positive cardiac myocytes. Inhibition of apoptosis by caspase inhibitors (i.e., ZVAD.fmk and DEVD.cmk) reduced I-123-AnxV myocardial uptake in LPS-treated rats. Eventually, endotoxin-treated rats displayed pathological uptake of Tc-99m-annexin in the cardiac mediastinal region whereas zVAD.fmk reduced Tc-99m-annexin mediastinal uptake. Our results show that radioactive I-123-AnxV signal emerging from LPS-treated rat hearts could be related to the activation of caspase-dependent apoptotic pathway in cardiac myocytes.}}, author = {{Petillot, Patrice and Lahorte, Christophe and Bonanno, Elena and Signore, Alberto and Lancel, Steve and Marchetti, Philippe and Vallet, Benoit and Slegers, Guido and Neviere, Remi}}, issn = {{1073-2322}}, journal = {{SHOCK}}, keywords = {{HEART-FAILURE,caspase inhibitors,IN-VITRO,PHOSPHATIDYLSERINE,SEPSIS,RATS,INFLAMMATION,REPERFUSION,INHIBITION,lipopolysaccharide,myocardial apoptosis,annexin V,MYOCYTE APOPTOSIS,MYOCARDIAL DYSFUNCTION}}, language = {{eng}}, number = {{1}}, pages = {{69--74}}, title = {{Annexin V detection of lipopolysaccharide-induced cardiac apoptosis}}, url = {{http://doi.org/10.1097/01.shk.0000235085.56100.38}}, volume = {{27}}, year = {{2007}}, }
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