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Thrombin generation in plasma of healthy adults and children: Chromogenic versus fluorogenic thrombogram analysis

Katrien Devreese (UGent) , Walter Wijns, Isabelle Combes, Soetkin Van kerckhoven and Marc F. Hoylaerts
(2007) THROMBOSIS AND HAEMOSTASIS. 98(3). p.600-613
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Abstract
Coagulation tests and coagulation factor assays have been complemented recently with experimental tests to measure the total amount of thrombin formed. We have presently analyzed thrombin generation of healthy adult and paediatric plasma samples via a fluorogenic and a chromogenic method. The chromogenic method was performed on the fully automated Behring Coagulation System((R)) (BCS (R)) and fluorogenic assays via Calibrated Automated Thrombography((R)) (CAT), after coagulation induction by various tissue factor (TF) concentrations. Sample distribution and variability were analyzed for the four main coagulation parameters, derived via computerized curve analysis in each method. Results for both methods were correlated. At the recommended TF concentration (300 pM),thrombin generation via BCS was less variable than via CAT (1-6 pM), but at comparable TF concentrations (1-6 pM), the CAT sensitivity was higher than that of BCS. Inhibition of intrinsic coagulation with the anti-factorVIII antibody BO2CII revealed that the BCS detected extrinsic coagulation exclusively, at all TF concentrations tested. In contrast, at lowTF concentrations (I and 2.5 pM), via CAT, intrinsic coagulation pathway amplification was measured. At standardized TF concentrations (300 pM in BCS vs. 2.5 pM in CAT), different reference values between adults and children were found, for all parameters, except Tmax. In adult samples, the best correlation between both methods was observed for ETPCAT versus ETPBCS and for Peak height(CAT) versus Cmax(BCS), when thrombin generation was exclusively extrinsic (300 pM in BCS vs. 6 pM in CAT). In conclusion, differential thrombin generation characteristics in BCS and CAT are relevant for their clinical applicability.
Keywords
tissue factor, SYSTEM, fibrinogen, coagulation assays, enclogenous thrombin potential

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MLA
Devreese, Katrien, et al. “Thrombin Generation in Plasma of Healthy Adults and Children: Chromogenic versus Fluorogenic Thrombogram Analysis.” THROMBOSIS AND HAEMOSTASIS, vol. 98, no. 3, 2007, pp. 600–13, doi:10.1160/TH07-03-0210.
APA
Devreese, K., Wijns, W., Combes, I., Van kerckhoven, S., & Hoylaerts, M. F. (2007). Thrombin generation in plasma of healthy adults and children: Chromogenic versus fluorogenic thrombogram analysis. THROMBOSIS AND HAEMOSTASIS, 98(3), 600–613. https://doi.org/10.1160/TH07-03-0210
Chicago author-date
Devreese, Katrien, Walter Wijns, Isabelle Combes, Soetkin Van kerckhoven, and Marc F. Hoylaerts. 2007. “Thrombin Generation in Plasma of Healthy Adults and Children: Chromogenic versus Fluorogenic Thrombogram Analysis.” THROMBOSIS AND HAEMOSTASIS 98 (3): 600–613. https://doi.org/10.1160/TH07-03-0210.
Chicago author-date (all authors)
Devreese, Katrien, Walter Wijns, Isabelle Combes, Soetkin Van kerckhoven, and Marc F. Hoylaerts. 2007. “Thrombin Generation in Plasma of Healthy Adults and Children: Chromogenic versus Fluorogenic Thrombogram Analysis.” THROMBOSIS AND HAEMOSTASIS 98 (3): 600–613. doi:10.1160/TH07-03-0210.
Vancouver
1.
Devreese K, Wijns W, Combes I, Van kerckhoven S, Hoylaerts MF. Thrombin generation in plasma of healthy adults and children: Chromogenic versus fluorogenic thrombogram analysis. THROMBOSIS AND HAEMOSTASIS. 2007;98(3):600–13.
IEEE
[1]
K. Devreese, W. Wijns, I. Combes, S. Van kerckhoven, and M. F. Hoylaerts, “Thrombin generation in plasma of healthy adults and children: Chromogenic versus fluorogenic thrombogram analysis,” THROMBOSIS AND HAEMOSTASIS, vol. 98, no. 3, pp. 600–613, 2007.
@article{743902,
  abstract     = {{Coagulation tests and coagulation factor assays have been complemented recently with experimental tests to measure the total amount of thrombin formed. We have presently analyzed thrombin generation of healthy adult and paediatric plasma samples via a fluorogenic and a chromogenic method. The chromogenic method was performed on the fully automated Behring Coagulation System((R)) (BCS (R)) and fluorogenic assays via Calibrated Automated Thrombography((R)) (CAT), after coagulation induction by various tissue factor (TF) concentrations. Sample distribution and variability were analyzed for the four main coagulation parameters, derived via computerized curve analysis in each method. Results for both methods were correlated. At the recommended TF concentration (300 pM),thrombin generation via BCS was less variable than via CAT (1-6 pM), but at comparable TF concentrations (1-6 pM), the CAT sensitivity was higher than that of BCS. Inhibition of intrinsic coagulation with the anti-factorVIII antibody BO2CII revealed that the BCS detected extrinsic coagulation exclusively, at all TF concentrations tested. In contrast, at lowTF concentrations (I and 2.5 pM), via CAT, intrinsic coagulation pathway amplification was measured. At standardized TF concentrations (300 pM in BCS vs. 2.5 pM in CAT), different reference values between adults and children were found, for all parameters, except Tmax. In adult samples, the best correlation between both methods was observed for ETPCAT versus ETPBCS and for Peak height(CAT) versus Cmax(BCS), when thrombin generation was exclusively extrinsic (300 pM in BCS vs. 6 pM in CAT). In conclusion, differential thrombin generation characteristics in BCS and CAT are relevant for their clinical applicability.}},
  author       = {{Devreese, Katrien and Wijns, Walter and Combes, Isabelle and Van kerckhoven, Soetkin and Hoylaerts, Marc F.}},
  issn         = {{0340-6245}},
  journal      = {{THROMBOSIS AND HAEMOSTASIS}},
  keywords     = {{tissue factor,SYSTEM,fibrinogen,coagulation assays,enclogenous thrombin potential}},
  language     = {{eng}},
  location     = {{Maastricht, Netherlands}},
  number       = {{3}},
  pages        = {{600--613}},
  title        = {{Thrombin generation in plasma of healthy adults and children: Chromogenic versus fluorogenic thrombogram analysis}},
  url          = {{http://doi.org/10.1160/TH07-03-0210}},
  volume       = {{98}},
  year         = {{2007}},
}
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