Prevalence and diversity of Salmonella serotypes in Ecuadorian broilers at slaughter age
- Author
- Christian Vinicio Vinueza Burgos (UGent) , Maria Cevallos, Lenin Ron-Garrido, Sophie Bertrand and Lieven De Zutter (UGent)
- Organization
- Abstract
- Salmonella is frequently found in poultry and represent an important source for human gastrointestinal infections worldwide. The aim of this study was to investigate the prevalence, genotypes and antimicrobial resistance of Salmonella serotypes in broilers from Ecuador. Caeca content from 388 at random selected broiler batches were collected in 6 slaughterhouses during 1 year and analyzed by the ISO 6579/Amd1 protocol for the isolation for Salmonella. Isolates were serotyped and genotypic variation was acceded by pulsed field gel electrophoresis. MIC values for sulfamethoxazole, gentamicin, ciprofloxacin, ampicillin, cefotaxime, ceftazidime, tetracycline, streptomycin, trimethropim, chloramphenicol, colistin, florfenicol, kanamycin and nalidixic acid were obtained. Presence of bla(CTX-M), bla(TEM), bla(SHV) and bla(CMY); and mcr-1 plasmid genes was investigated in resistant strains to cefotaxime and colistin respectively. Prevalence at batch level was 16.0%. The most common serotype was S. Infantis (83.9%) followed by S. Enteritidis (14.5%) and S. Corvallis (1.6%). The pulsed field gel electrophoresis analysis showed that S. Corvallis, S. Enteritidis and S. Infantis isolates belonged to 1, 2 and 12 genotypes respectively. S. Infantis isolates showed high resistance rates to 12 antibiotics ranging from 57.7%(kanamycin) up to 98.1% (nalidixic acid and sulfamethoxazole). All S. Enteritidis isolates showed resistance to colistin. High multiresistant patterns were found for all the serotypes. The bla(CTX-M) gene was present in 33 S. Infantis isolates while mcr-1 was negative in 10 colistin resistant isolates. This study provides the first set of scientific data on prevalence and multidrug-resistant Salmonella coming from commercial poultry in Ecuador.
- Keywords
- SPECTRUM BETA-LACTAMASE, RESISTANT ESCHERICHIA-COLI, ENTERICA SEROVAR INFANTIS, FIELD GEL-ELECTROPHORESIS, ANTIMICROBIAL RESISTANCE, ANTIBIOTIC-RESISTANCE, FOOD ANIMALS, CHICKEN MEAT, POULTRY, SUSCEPTIBILITY
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8110266
- MLA
- Vinueza Burgos, Christian Vinicio, et al. “Prevalence and Diversity of Salmonella Serotypes in Ecuadorian Broilers at Slaughter Age.” PLOS ONE, vol. 11, no. 7, 2016, doi:10.1371/journal.pone.0159567.
- APA
- Vinueza Burgos, C. V., Cevallos, M., Ron-Garrido, L., Bertrand, S., & De Zutter, L. (2016). Prevalence and diversity of Salmonella serotypes in Ecuadorian broilers at slaughter age. PLOS ONE, 11(7). https://doi.org/10.1371/journal.pone.0159567
- Chicago author-date
- Vinueza Burgos, Christian Vinicio, Maria Cevallos, Lenin Ron-Garrido, Sophie Bertrand, and Lieven De Zutter. 2016. “Prevalence and Diversity of Salmonella Serotypes in Ecuadorian Broilers at Slaughter Age.” PLOS ONE 11 (7). https://doi.org/10.1371/journal.pone.0159567.
- Chicago author-date (all authors)
- Vinueza Burgos, Christian Vinicio, Maria Cevallos, Lenin Ron-Garrido, Sophie Bertrand, and Lieven De Zutter. 2016. “Prevalence and Diversity of Salmonella Serotypes in Ecuadorian Broilers at Slaughter Age.” PLOS ONE 11 (7). doi:10.1371/journal.pone.0159567.
- Vancouver
- 1.Vinueza Burgos CV, Cevallos M, Ron-Garrido L, Bertrand S, De Zutter L. Prevalence and diversity of Salmonella serotypes in Ecuadorian broilers at slaughter age. PLOS ONE. 2016;11(7).
- IEEE
- [1]C. V. Vinueza Burgos, M. Cevallos, L. Ron-Garrido, S. Bertrand, and L. De Zutter, “Prevalence and diversity of Salmonella serotypes in Ecuadorian broilers at slaughter age,” PLOS ONE, vol. 11, no. 7, 2016.
@article{8110266, abstract = {{Salmonella is frequently found in poultry and represent an important source for human gastrointestinal infections worldwide. The aim of this study was to investigate the prevalence, genotypes and antimicrobial resistance of Salmonella serotypes in broilers from Ecuador. Caeca content from 388 at random selected broiler batches were collected in 6 slaughterhouses during 1 year and analyzed by the ISO 6579/Amd1 protocol for the isolation for Salmonella. Isolates were serotyped and genotypic variation was acceded by pulsed field gel electrophoresis. MIC values for sulfamethoxazole, gentamicin, ciprofloxacin, ampicillin, cefotaxime, ceftazidime, tetracycline, streptomycin, trimethropim, chloramphenicol, colistin, florfenicol, kanamycin and nalidixic acid were obtained. Presence of bla(CTX-M), bla(TEM), bla(SHV) and bla(CMY); and mcr-1 plasmid genes was investigated in resistant strains to cefotaxime and colistin respectively. Prevalence at batch level was 16.0%. The most common serotype was S. Infantis (83.9%) followed by S. Enteritidis (14.5%) and S. Corvallis (1.6%). The pulsed field gel electrophoresis analysis showed that S. Corvallis, S. Enteritidis and S. Infantis isolates belonged to 1, 2 and 12 genotypes respectively. S. Infantis isolates showed high resistance rates to 12 antibiotics ranging from 57.7%(kanamycin) up to 98.1% (nalidixic acid and sulfamethoxazole). All S. Enteritidis isolates showed resistance to colistin. High multiresistant patterns were found for all the serotypes. The bla(CTX-M) gene was present in 33 S. Infantis isolates while mcr-1 was negative in 10 colistin resistant isolates. This study provides the first set of scientific data on prevalence and multidrug-resistant Salmonella coming from commercial poultry in Ecuador.}}, articleno = {{e0159567}}, author = {{Vinueza Burgos, Christian Vinicio and Cevallos, Maria and Ron-Garrido, Lenin and Bertrand, Sophie and De Zutter, Lieven}}, issn = {{1932-6203}}, journal = {{PLOS ONE}}, keywords = {{SPECTRUM BETA-LACTAMASE,RESISTANT ESCHERICHIA-COLI,ENTERICA SEROVAR INFANTIS,FIELD GEL-ELECTROPHORESIS,ANTIMICROBIAL RESISTANCE,ANTIBIOTIC-RESISTANCE,FOOD ANIMALS,CHICKEN MEAT,POULTRY,SUSCEPTIBILITY}}, language = {{eng}}, number = {{7}}, pages = {{12}}, title = {{Prevalence and diversity of Salmonella serotypes in Ecuadorian broilers at slaughter age}}, url = {{http://doi.org/10.1371/journal.pone.0159567}}, volume = {{11}}, year = {{2016}}, }
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