Toxicity, membrane binding and uptake of the Sclerotinia sclerotiorum agglutinin (SSA) in different insect cell lines
- Author
- Ying Shen (UGent) , Kristof De Schutter (UGent) , Tomasz Walski (UGent) , Els Van Damme (UGent) and Guy Smagghe (UGent)
- Organization
- Abstract
- The fungal lectin purified from Sclerotinia sclerotiorum, further referred to as Sclerotinia sclerotiorum agglutinin or SSA, possesses insecticidal activity against important pest insects such as pea aphids (Acyrthosiphon pisum). This paper aims at a better understanding of its activity at cellular level. Therefore, different insect cell lines were treated with SSA. These cell lines were derived from different tissues and represent the three major orders of insects important in agriculture: CF-203 (midgut Choristoneura fumiferana, Lepidoptera), GUTAW1 (midgut, Helicoverpa zea, Lepidoptera), High5 cells (ovary, Trichoplusia ni, Lepidoptera), Sf9 (ovary cells from Spodoptera frugiperda, Lepidoptera), S2 (hemocyte, Drosophila melanogaster, Diptera), and TcA (whole body, Tribolium castaneum, Coleoptera). Although the sensitivity to SSA differs between the cell lines, SSA clearly showed toxicity in all six cell lines with median effect concentrations (EC50) ranging between 9 and 42 mu g/ml. An in-depth analysis of the mechanism of uptake in the cells revealed superior amounts of FITC-SSA at the membrane of CF-203 cells compared to Sf9 cells, while a similar small amount of SSA was internalized in both cell lines. Pre-incubation with the clathrin-mediated endocytosis inhibitor phenylarsine oxide inhibited the internalization of SSA into the CF-203 and Sf9 cells with a respective reduction of 6- and 1.7-fold. The data are discussed in relation to the importance of cellular uptake mechanism for SSA binding and cytotoxicity.
- Keywords
- Insect cell, Sclerotinia sclerotiorum agglutinin, Cytotoxicity, Localization, Endocytosis, BEETLE TRIBOLIUM-CASTANEUM, SPODOPTERA-LITTORALIS, PLANT-LECTINS, ALKALINE-PHOSPHATASE, RHIZOCTONIA-SOLANI, SNOWDROP LECTIN, MECHANISM, TOXIN, GLYCOSYLATION, PROTEINS
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8540746
- MLA
- Shen, Ying, et al. “Toxicity, Membrane Binding and Uptake of the Sclerotinia Sclerotiorum Agglutinin (SSA) in Different Insect Cell Lines.” IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, vol. 53, no. 8, 2017, pp. 691–98, doi:10.1007/s11626-017-0176-8.
- APA
- Shen, Y., De Schutter, K., Walski, T., Van Damme, E., & Smagghe, G. (2017). Toxicity, membrane binding and uptake of the Sclerotinia sclerotiorum agglutinin (SSA) in different insect cell lines. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 53(8), 691–698. https://doi.org/10.1007/s11626-017-0176-8
- Chicago author-date
- Shen, Ying, Kristof De Schutter, Tomasz Walski, Els Van Damme, and Guy Smagghe. 2017. “Toxicity, Membrane Binding and Uptake of the Sclerotinia Sclerotiorum Agglutinin (SSA) in Different Insect Cell Lines.” IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 53 (8): 691–98. https://doi.org/10.1007/s11626-017-0176-8.
- Chicago author-date (all authors)
- Shen, Ying, Kristof De Schutter, Tomasz Walski, Els Van Damme, and Guy Smagghe. 2017. “Toxicity, Membrane Binding and Uptake of the Sclerotinia Sclerotiorum Agglutinin (SSA) in Different Insect Cell Lines.” IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 53 (8): 691–698. doi:10.1007/s11626-017-0176-8.
- Vancouver
- 1.Shen Y, De Schutter K, Walski T, Van Damme E, Smagghe G. Toxicity, membrane binding and uptake of the Sclerotinia sclerotiorum agglutinin (SSA) in different insect cell lines. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL. 2017;53(8):691–8.
- IEEE
- [1]Y. Shen, K. De Schutter, T. Walski, E. Van Damme, and G. Smagghe, “Toxicity, membrane binding and uptake of the Sclerotinia sclerotiorum agglutinin (SSA) in different insect cell lines,” IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, vol. 53, no. 8, pp. 691–698, 2017.
@article{8540746, abstract = {{The fungal lectin purified from Sclerotinia sclerotiorum, further referred to as Sclerotinia sclerotiorum agglutinin or SSA, possesses insecticidal activity against important pest insects such as pea aphids (Acyrthosiphon pisum). This paper aims at a better understanding of its activity at cellular level. Therefore, different insect cell lines were treated with SSA. These cell lines were derived from different tissues and represent the three major orders of insects important in agriculture: CF-203 (midgut Choristoneura fumiferana, Lepidoptera), GUTAW1 (midgut, Helicoverpa zea, Lepidoptera), High5 cells (ovary, Trichoplusia ni, Lepidoptera), Sf9 (ovary cells from Spodoptera frugiperda, Lepidoptera), S2 (hemocyte, Drosophila melanogaster, Diptera), and TcA (whole body, Tribolium castaneum, Coleoptera). Although the sensitivity to SSA differs between the cell lines, SSA clearly showed toxicity in all six cell lines with median effect concentrations (EC50) ranging between 9 and 42 mu g/ml. An in-depth analysis of the mechanism of uptake in the cells revealed superior amounts of FITC-SSA at the membrane of CF-203 cells compared to Sf9 cells, while a similar small amount of SSA was internalized in both cell lines. Pre-incubation with the clathrin-mediated endocytosis inhibitor phenylarsine oxide inhibited the internalization of SSA into the CF-203 and Sf9 cells with a respective reduction of 6- and 1.7-fold. The data are discussed in relation to the importance of cellular uptake mechanism for SSA binding and cytotoxicity.}}, author = {{Shen, Ying and De Schutter, Kristof and Walski, Tomasz and Van Damme, Els and Smagghe, Guy}}, issn = {{1071-2690}}, journal = {{IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL}}, keywords = {{Insect cell,Sclerotinia sclerotiorum agglutinin,Cytotoxicity,Localization,Endocytosis,BEETLE TRIBOLIUM-CASTANEUM,SPODOPTERA-LITTORALIS,PLANT-LECTINS,ALKALINE-PHOSPHATASE,RHIZOCTONIA-SOLANI,SNOWDROP LECTIN,MECHANISM,TOXIN,GLYCOSYLATION,PROTEINS}}, language = {{eng}}, number = {{8}}, pages = {{691--698}}, title = {{Toxicity, membrane binding and uptake of the Sclerotinia sclerotiorum agglutinin (SSA) in different insect cell lines}}, url = {{http://doi.org/10.1007/s11626-017-0176-8}}, volume = {{53}}, year = {{2017}}, }
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