Advanced search
1 file | 658.99 KB Add to list

The N-glycan profile of the peritrophic membrane in the Colorado potato beetle larva (Leptinotarsa decemlineata)

Author
Organization
Abstract
The insect peritrophic membrane (PM) is a non-cellular structure composed of secreted proteins imbedded in a proteoglycan matrix together with chitin. It separates the midgut epithelium from the intestinal contents, and functions in the digestion of food. Furthermore it acts as a protective barrier against abrasive particles and microbial infections. Here we studied for the first time the N-glycome of the PM. We identified the N-glycan structures present in the PM of the Colorado potato beetle (CPB) at the fourth larval stage using MALDI-TOF mass spectrometry. In parallel, we correlated the N-glycan data to the presence of the N-glycosylation related genes (NGRGs) in the transcriptome of epithelial midgut cells. The presumed activities of the identified genes support the N-glycan profile resolved for the proteins in the PM. To our knowledge these data are the first report on the N-glycome of the PM of a pest insect. These results will contribute to the study of the importance of N-glycosylation in the function and structure of the PM. In addition, the data can help to find novel targets and design better biopesticides for pest control.
Keywords
N-glycosylation, N-glycome, Leptinotarsa decemlineata, Midgut, Transcriptome, Peritrophic membrane, LUCILIA-CUPRINA, GLYCOSYLATED PROTEINS, MATRIX, IDENTIFICATION, COLEOPTERA

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 658.99 KB

Citation

Please use this url to cite or link to this publication:

MLA
Liu, Dongdong, et al. “The N-Glycan Profile of the Peritrophic Membrane in the Colorado Potato Beetle Larva (Leptinotarsa Decemlineata).” JOURNAL OF INSECT PHYSIOLOGY, vol. 115, 2019, pp. 27–32, doi:10.1016/j.jinsphys.2019.03.009.
APA
Liu, D., De Schutter, K., Smargiasso, N., De Pauw, E., Van Damme, E., & Smagghe, G. (2019). The N-glycan profile of the peritrophic membrane in the Colorado potato beetle larva (Leptinotarsa decemlineata). JOURNAL OF INSECT PHYSIOLOGY, 115, 27–32. https://doi.org/10.1016/j.jinsphys.2019.03.009
Chicago author-date
Liu, Dongdong, Kristof De Schutter, Nicolas Smargiasso, Edwin De Pauw, Els Van Damme, and Guy Smagghe. 2019. “The N-Glycan Profile of the Peritrophic Membrane in the Colorado Potato Beetle Larva (Leptinotarsa Decemlineata).” JOURNAL OF INSECT PHYSIOLOGY 115: 27–32. https://doi.org/10.1016/j.jinsphys.2019.03.009.
Chicago author-date (all authors)
Liu, Dongdong, Kristof De Schutter, Nicolas Smargiasso, Edwin De Pauw, Els Van Damme, and Guy Smagghe. 2019. “The N-Glycan Profile of the Peritrophic Membrane in the Colorado Potato Beetle Larva (Leptinotarsa Decemlineata).” JOURNAL OF INSECT PHYSIOLOGY 115: 27–32. doi:10.1016/j.jinsphys.2019.03.009.
Vancouver
1.
Liu D, De Schutter K, Smargiasso N, De Pauw E, Van Damme E, Smagghe G. The N-glycan profile of the peritrophic membrane in the Colorado potato beetle larva (Leptinotarsa decemlineata). JOURNAL OF INSECT PHYSIOLOGY. 2019;115:27–32.
IEEE
[1]
D. Liu, K. De Schutter, N. Smargiasso, E. De Pauw, E. Van Damme, and G. Smagghe, “The N-glycan profile of the peritrophic membrane in the Colorado potato beetle larva (Leptinotarsa decemlineata),” JOURNAL OF INSECT PHYSIOLOGY, vol. 115, pp. 27–32, 2019.
@article{8619827,
  abstract     = {{The insect peritrophic membrane (PM) is a non-cellular structure composed of secreted proteins imbedded in a proteoglycan matrix together with chitin. It separates the midgut epithelium from the intestinal contents, and functions in the digestion of food. Furthermore it acts as a protective barrier against abrasive particles and microbial infections. Here we studied for the first time the N-glycome of the PM. We identified the N-glycan structures present in the PM of the Colorado potato beetle (CPB) at the fourth larval stage using MALDI-TOF mass spectrometry. In parallel, we correlated the N-glycan data to the presence of the N-glycosylation related genes (NGRGs) in the transcriptome of epithelial midgut cells. The presumed activities of the identified genes support the N-glycan profile resolved for the proteins in the PM. To our knowledge these data are the first report on the N-glycome of the PM of a pest insect. These results will contribute to the study of the importance of N-glycosylation in the function and structure of the PM. In addition, the data can help to find novel targets and design better biopesticides for pest control.}},
  author       = {{Liu, Dongdong and De Schutter, Kristof and Smargiasso, Nicolas and De Pauw, Edwin and Van Damme, Els and Smagghe, Guy}},
  issn         = {{0022-1910}},
  journal      = {{JOURNAL OF INSECT PHYSIOLOGY}},
  keywords     = {{N-glycosylation,N-glycome,Leptinotarsa decemlineata,Midgut,Transcriptome,Peritrophic membrane,LUCILIA-CUPRINA,GLYCOSYLATED PROTEINS,MATRIX,IDENTIFICATION,COLEOPTERA}},
  language     = {{eng}},
  pages        = {{27--32}},
  title        = {{The N-glycan profile of the peritrophic membrane in the Colorado potato beetle larva (Leptinotarsa decemlineata)}},
  url          = {{http://doi.org/10.1016/j.jinsphys.2019.03.009}},
  volume       = {{115}},
  year         = {{2019}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: