An anticaffeine antibody-oligonucleotide conjugate for DNA-directed immobilization in environmental immunoarrays
- Author
- Ana Margarida Carvalho, Cinthya Veliz Montes, Rudolf J. Schneider and Annemieke Madder (UGent)
- Organization
- Abstract
- The development of fast and cheap high-throughput platforms for the detection of environmental contaminants is of particular importance to understand the human-related impact on the environment. The application of DNA-directed immobilization (DDI) of IgG molecules is currently limited to the clinical diagnostics scenario, possibly because of the high costs of production of such addressable platforms. We here describe the efficient and specific hybridization of an antibody-oligonucleotide conjugate to a short 12-mer capture probe. The specific antibody used is a monoclonal antibody against caffeine, a stimulant and important anthropogenic marker. With this work, we hope to contribute to broadening the application potential of DDI to environmental markers in order to develop cheaper and more stable high-throughput screening platforms for standard routine analysis of pollutants in a variety of complex matrices.
- Keywords
- MICROARRAY TECHNOLOGY, FRAGMENTATION, CARBAMAZEPINE, CAFFEINE, ELISA
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8714071
- MLA
- Carvalho, Ana Margarida, et al. “An Anticaffeine Antibody-Oligonucleotide Conjugate for DNA-Directed Immobilization in Environmental Immunoarrays.” LANGMUIR, vol. 34, no. 49, 2018, pp. 14834–41, doi:10.1021/acs.langmuir.8b01347.
- APA
- Carvalho, A. M., Montes, C. V., Schneider, R. J., & Madder, A. (2018). An anticaffeine antibody-oligonucleotide conjugate for DNA-directed immobilization in environmental immunoarrays. LANGMUIR, 34(49), 14834–14841. https://doi.org/10.1021/acs.langmuir.8b01347
- Chicago author-date
- Carvalho, Ana Margarida, Cinthya Veliz Montes, Rudolf J. Schneider, and Annemieke Madder. 2018. “An Anticaffeine Antibody-Oligonucleotide Conjugate for DNA-Directed Immobilization in Environmental Immunoarrays.” LANGMUIR 34 (49): 14834–41. https://doi.org/10.1021/acs.langmuir.8b01347.
- Chicago author-date (all authors)
- Carvalho, Ana Margarida, Cinthya Veliz Montes, Rudolf J. Schneider, and Annemieke Madder. 2018. “An Anticaffeine Antibody-Oligonucleotide Conjugate for DNA-Directed Immobilization in Environmental Immunoarrays.” LANGMUIR 34 (49): 14834–14841. doi:10.1021/acs.langmuir.8b01347.
- Vancouver
- 1.Carvalho AM, Montes CV, Schneider RJ, Madder A. An anticaffeine antibody-oligonucleotide conjugate for DNA-directed immobilization in environmental immunoarrays. LANGMUIR. 2018;34(49):14834–41.
- IEEE
- [1]A. M. Carvalho, C. V. Montes, R. J. Schneider, and A. Madder, “An anticaffeine antibody-oligonucleotide conjugate for DNA-directed immobilization in environmental immunoarrays,” LANGMUIR, vol. 34, no. 49, pp. 14834–14841, 2018.
@article{8714071,
abstract = {{The development of fast and cheap high-throughput platforms for the detection of environmental contaminants is of particular importance to understand the human-related impact on the environment. The application of DNA-directed immobilization (DDI) of IgG molecules is currently limited to the clinical diagnostics scenario, possibly because of the high costs of production of such addressable platforms. We here describe the efficient and specific hybridization of an antibody-oligonucleotide conjugate to a short 12-mer capture probe. The specific antibody used is a monoclonal antibody against caffeine, a stimulant and important anthropogenic marker. With this work, we hope to contribute to broadening the application potential of DDI to environmental markers in order to develop cheaper and more stable high-throughput screening platforms for standard routine analysis of pollutants in a variety of complex matrices.}},
author = {{Carvalho, Ana Margarida and Montes, Cinthya Veliz and Schneider, Rudolf J. and Madder, Annemieke}},
issn = {{0743-7463}},
journal = {{LANGMUIR}},
keywords = {{MICROARRAY TECHNOLOGY,FRAGMENTATION,CARBAMAZEPINE,CAFFEINE,ELISA}},
language = {{eng}},
number = {{49}},
pages = {{14834--14841}},
title = {{An anticaffeine antibody-oligonucleotide conjugate for DNA-directed immobilization in environmental immunoarrays}},
url = {{http://doi.org/10.1021/acs.langmuir.8b01347}},
volume = {{34}},
year = {{2018}},
}
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