RNAi of the N-glycosylation-related genes confirms their importance in insect development and alpha-1,6-fucosyltransferase plays a role in the ecdysis event for the hemimetabolous pest insect Nilaparvata lugens
- Author
- Qun Yang (UGent) , Kristof De Schutter (UGent) , Pengyu Chen, Els Van Damme (UGent) and Guy Smagghe (UGent)
- Organization
- Abstract
- Recently N-glycosylation was found to be required for the postembryonic development and metamorphosis of the holometabolous beetle Tribolium castaneum. However, the role of N-glycosylation in the development of hemimetabolous insects is unknown. To further elucidate the role of N-glycosylation in the development of insects, a functional characterization of the N-glycosylation-related genes (NGRGs) was performed in a model insect for hemimetabolous development, namely the brown planthopper Nilaparvata lugens. In this project, we report the effects of RNAi-mediated silencing of 15 NGRGs on the postembryonic development of N. lugens. Two major observations were made. First, interruption of the early steps of N-glycan processing led to a lethal phenotype during the transition from nymph to adult as was observed in T. castaneum. Second, we report here on an essential function for the alpha-1,6-fucosyl transferase in the ecdysis event of N. lugens between nymphal instars, since gene-silencing by RNAi led to failure of ecdysis and subsequent mortality of the treated insect.
- Keywords
- development, glycosylation, Nilaparvata lugens, N‐, glycan, postembryonic, RNA interference
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8714146
- MLA
- Yang, Qun, et al. “RNAi of the N-Glycosylation-Related Genes Confirms Their Importance in Insect Development and Alpha-1,6-Fucosyltransferase Plays a Role in the Ecdysis Event for the Hemimetabolous Pest Insect Nilaparvata Lugens.” INSECT SCIENCE, vol. 29, no. 1, 2022, pp. 91–99, doi:10.1111/1744-7917.12920.
- APA
- Yang, Q., De Schutter, K., Chen, P., Van Damme, E., & Smagghe, G. (2022). RNAi of the N-glycosylation-related genes confirms their importance in insect development and alpha-1,6-fucosyltransferase plays a role in the ecdysis event for the hemimetabolous pest insect Nilaparvata lugens. INSECT SCIENCE, 29(1), 91–99. https://doi.org/10.1111/1744-7917.12920
- Chicago author-date
- Yang, Qun, Kristof De Schutter, Pengyu Chen, Els Van Damme, and Guy Smagghe. 2022. “RNAi of the N-Glycosylation-Related Genes Confirms Their Importance in Insect Development and Alpha-1,6-Fucosyltransferase Plays a Role in the Ecdysis Event for the Hemimetabolous Pest Insect Nilaparvata Lugens.” INSECT SCIENCE 29 (1): 91–99. https://doi.org/10.1111/1744-7917.12920.
- Chicago author-date (all authors)
- Yang, Qun, Kristof De Schutter, Pengyu Chen, Els Van Damme, and Guy Smagghe. 2022. “RNAi of the N-Glycosylation-Related Genes Confirms Their Importance in Insect Development and Alpha-1,6-Fucosyltransferase Plays a Role in the Ecdysis Event for the Hemimetabolous Pest Insect Nilaparvata Lugens.” INSECT SCIENCE 29 (1): 91–99. doi:10.1111/1744-7917.12920.
- Vancouver
- 1.Yang Q, De Schutter K, Chen P, Van Damme E, Smagghe G. RNAi of the N-glycosylation-related genes confirms their importance in insect development and alpha-1,6-fucosyltransferase plays a role in the ecdysis event for the hemimetabolous pest insect Nilaparvata lugens. INSECT SCIENCE. 2022;29(1):91–9.
- IEEE
- [1]Q. Yang, K. De Schutter, P. Chen, E. Van Damme, and G. Smagghe, “RNAi of the N-glycosylation-related genes confirms their importance in insect development and alpha-1,6-fucosyltransferase plays a role in the ecdysis event for the hemimetabolous pest insect Nilaparvata lugens,” INSECT SCIENCE, vol. 29, no. 1, pp. 91–99, 2022.
@article{8714146, abstract = {{Recently N-glycosylation was found to be required for the postembryonic development and metamorphosis of the holometabolous beetle Tribolium castaneum. However, the role of N-glycosylation in the development of hemimetabolous insects is unknown. To further elucidate the role of N-glycosylation in the development of insects, a functional characterization of the N-glycosylation-related genes (NGRGs) was performed in a model insect for hemimetabolous development, namely the brown planthopper Nilaparvata lugens. In this project, we report the effects of RNAi-mediated silencing of 15 NGRGs on the postembryonic development of N. lugens. Two major observations were made. First, interruption of the early steps of N-glycan processing led to a lethal phenotype during the transition from nymph to adult as was observed in T. castaneum. Second, we report here on an essential function for the alpha-1,6-fucosyl transferase in the ecdysis event of N. lugens between nymphal instars, since gene-silencing by RNAi led to failure of ecdysis and subsequent mortality of the treated insect.}}, author = {{Yang, Qun and De Schutter, Kristof and Chen, Pengyu and Van Damme, Els and Smagghe, Guy}}, issn = {{1672-9609}}, journal = {{INSECT SCIENCE}}, keywords = {{development,glycosylation,Nilaparvata lugens,N‐,glycan,postembryonic,RNA interference}}, language = {{eng}}, number = {{1}}, pages = {{91--99}}, title = {{RNAi of the N-glycosylation-related genes confirms their importance in insect development and alpha-1,6-fucosyltransferase plays a role in the ecdysis event for the hemimetabolous pest insect Nilaparvata lugens}}, url = {{http://doi.org/10.1111/1744-7917.12920}}, volume = {{29}}, year = {{2022}}, }
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