Modeling and quantification of cancer cell invasion through collagen type I matrices
- Author
- Olivier De Wever (UGent) , An Hendrix (UGent) , Astrid De Boeck (UGent) , Wendy Westbroek, Geert Braems (UGent) , Shahin Emami, Michèle Sabbah, Christian Gespach and Marc Bracke (UGent)
- Organization
- Abstract
- Tumor invasion is the outcome of a complex interplay between cancer cells and the stromal environment. Considering the contribution of the stromal environment, we developed a membrane-free single-cell and spheroid based complementary model to study cancer invasion through native collagen type-I matrices. Cell morphology is preserved during the assays allowing real time monitoring of invasion-induced changes in cell structure and F-actin organization. Combining these models with computerized quantification permits the calculation of highly reproducible and operator-independent data. These assays are versatile in the use of fluorescent probes and have a flexible kinetic endpoint. Once the optimal experimental conditions are empirically determined, the collagen type-I invasion assays can be used for preclinical validation of small-molecule inhibitors targeting invasion. Initiation and monitoring of the single-cell and spheroid invasion model can be achieved in 8 h (over 3 days) and in 14 h (over 8 days) respectively.
- Keywords
- collagen, invasion, stroma, 3D matrices, ecosystem, BREAST-CANCER, SIGNALING PATHWAYS, GROWTH-FACTOR, TUMOR-CELLS, N-CADHERIN, TGF-BETA, VITRO, DIFFERENTIATION, GENE, MICROENVIRONMENT
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-872838
- MLA
- De Wever, Olivier, et al. “Modeling and Quantification of Cancer Cell Invasion through Collagen Type I Matrices.” INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, vol. 54, no. 5, 2010, pp. 887–96, doi:10.1387/ijdb.092948ow.
- APA
- De Wever, O., Hendrix, A., De Boeck, A., Westbroek, W., Braems, G., Emami, S., … Bracke, M. (2010). Modeling and quantification of cancer cell invasion through collagen type I matrices. INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, 54(5), 887–896. https://doi.org/10.1387/ijdb.092948ow
- Chicago author-date
- De Wever, Olivier, An Hendrix, Astrid De Boeck, Wendy Westbroek, Geert Braems, Shahin Emami, Michèle Sabbah, Christian Gespach, and Marc Bracke. 2010. “Modeling and Quantification of Cancer Cell Invasion through Collagen Type I Matrices.” INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY 54 (5): 887–96. https://doi.org/10.1387/ijdb.092948ow.
- Chicago author-date (all authors)
- De Wever, Olivier, An Hendrix, Astrid De Boeck, Wendy Westbroek, Geert Braems, Shahin Emami, Michèle Sabbah, Christian Gespach, and Marc Bracke. 2010. “Modeling and Quantification of Cancer Cell Invasion through Collagen Type I Matrices.” INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY 54 (5): 887–896. doi:10.1387/ijdb.092948ow.
- Vancouver
- 1.De Wever O, Hendrix A, De Boeck A, Westbroek W, Braems G, Emami S, et al. Modeling and quantification of cancer cell invasion through collagen type I matrices. INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY. 2010;54(5):887–96.
- IEEE
- [1]O. De Wever et al., “Modeling and quantification of cancer cell invasion through collagen type I matrices,” INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, vol. 54, no. 5, pp. 887–896, 2010.
@article{872838, abstract = {{Tumor invasion is the outcome of a complex interplay between cancer cells and the stromal environment. Considering the contribution of the stromal environment, we developed a membrane-free single-cell and spheroid based complementary model to study cancer invasion through native collagen type-I matrices. Cell morphology is preserved during the assays allowing real time monitoring of invasion-induced changes in cell structure and F-actin organization. Combining these models with computerized quantification permits the calculation of highly reproducible and operator-independent data. These assays are versatile in the use of fluorescent probes and have a flexible kinetic endpoint. Once the optimal experimental conditions are empirically determined, the collagen type-I invasion assays can be used for preclinical validation of small-molecule inhibitors targeting invasion. Initiation and monitoring of the single-cell and spheroid invasion model can be achieved in 8 h (over 3 days) and in 14 h (over 8 days) respectively.}}, author = {{De Wever, Olivier and Hendrix, An and De Boeck, Astrid and Westbroek, Wendy and Braems, Geert and Emami, Shahin and Sabbah, Michèle and Gespach, Christian and Bracke, Marc}}, issn = {{1696-3547}}, journal = {{INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY}}, keywords = {{collagen,invasion,stroma,3D matrices,ecosystem,BREAST-CANCER,SIGNALING PATHWAYS,GROWTH-FACTOR,TUMOR-CELLS,N-CADHERIN,TGF-BETA,VITRO,DIFFERENTIATION,GENE,MICROENVIRONMENT}}, language = {{eng}}, number = {{5}}, pages = {{887--896}}, title = {{Modeling and quantification of cancer cell invasion through collagen type I matrices}}, url = {{http://doi.org/10.1387/ijdb.092948ow}}, volume = {{54}}, year = {{2010}}, }
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