Comparison of capillary electrophoresis and zwitterionic-hydrophilic interaction capillary liquid chromatography with ultraviolet and mass spectrometry detection for the analysis of microRNA biomarkers
- Author
- Jeanneth Vasconez, Roger Peró Gascón (UGent) , Estela Gimenez and Fernando Benavente
- Organization
- Abstract
- This study evaluates zwitterionic-hydrophilic interaction capillary liquid chromatography (capZIC-HILIC) and capillary electrophoresis (CE) with ultraviolet (UV) and mass spectrometry (MS) detection for the direct, label free and multiplex analysis of microribonucleic acids (miRNAs). CapZIC-HILIC-UV and CE-UV methods were first optimized, resulting in similar separations for a mixture of three miRNAs (hsa-iso-miR-16-5p, hsa-let-7g-5p, and hsa-miR-21-5p) but with reversal of elution/migration orders and small differences in repeatability, linearity, limit of detection (LOD) and separation efficiency. The established UV methods were transferred and validated in these terms with mass spectrometry (MS) detection, which allowed identifying the miRNAs and characterizing their post-transcriptional modifications. LOD by capZIC-HILIC-MS was 1 mu M of miRNA, around 5 times lower than by CE-MS due to the analyte dilution with the sheathflow CE-MS interface and to the slightly increased abundance of alkali metals adducts in the CE-MS mass spectra. In addition, the suction effect promoted by the nebulizer gas in CE-MS negatively affected the already compromised separations. In contrast, CE-MS showed superior repeatabilities with spiked serum samples, as well as reduced costs, extended capillary column durabilities and shorter conditioning times. The comparison of the different methods allows disclosing the current advantages and disadvantages of capZIC-HILIC and CE for the analysis of miRNA biomarkers.
- Keywords
- Biomarker, Capillary electrophoresis, Capillary liquid chromatography, Mass spectrometry, miRNA, ZIC-HILIC, SERUM, OLIGONUCLEOTIDES, RETENTION, MIRNA, TIME
Downloads
-
CapZICHILIC vs CE miRNAs postprint.pdf
- full text (Accepted manuscript)
- |
- open access
- |
- |
- 1.12 MB
-
(...).pdf
- full text (Published version)
- |
- UGent only
- |
- |
- 1.15 MB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8732241
- MLA
- Vasconez, Jeanneth, et al. “Comparison of Capillary Electrophoresis and Zwitterionic-Hydrophilic Interaction Capillary Liquid Chromatography with Ultraviolet and Mass Spectrometry Detection for the Analysis of MicroRNA Biomarkers.” TALANTA, vol. 219, 2020, doi:10.1016/j.talanta.2020.121263.
- APA
- Vasconez, J., Peró Gascón, R., Gimenez, E., & Benavente, F. (2020). Comparison of capillary electrophoresis and zwitterionic-hydrophilic interaction capillary liquid chromatography with ultraviolet and mass spectrometry detection for the analysis of microRNA biomarkers. TALANTA, 219. https://doi.org/10.1016/j.talanta.2020.121263
- Chicago author-date
- Vasconez, Jeanneth, Roger Peró Gascón, Estela Gimenez, and Fernando Benavente. 2020. “Comparison of Capillary Electrophoresis and Zwitterionic-Hydrophilic Interaction Capillary Liquid Chromatography with Ultraviolet and Mass Spectrometry Detection for the Analysis of MicroRNA Biomarkers.” TALANTA 219. https://doi.org/10.1016/j.talanta.2020.121263.
- Chicago author-date (all authors)
- Vasconez, Jeanneth, Roger Peró Gascón, Estela Gimenez, and Fernando Benavente. 2020. “Comparison of Capillary Electrophoresis and Zwitterionic-Hydrophilic Interaction Capillary Liquid Chromatography with Ultraviolet and Mass Spectrometry Detection for the Analysis of MicroRNA Biomarkers.” TALANTA 219. doi:10.1016/j.talanta.2020.121263.
- Vancouver
- 1.Vasconez J, Peró Gascón R, Gimenez E, Benavente F. Comparison of capillary electrophoresis and zwitterionic-hydrophilic interaction capillary liquid chromatography with ultraviolet and mass spectrometry detection for the analysis of microRNA biomarkers. TALANTA. 2020;219.
- IEEE
- [1]J. Vasconez, R. Peró Gascón, E. Gimenez, and F. Benavente, “Comparison of capillary electrophoresis and zwitterionic-hydrophilic interaction capillary liquid chromatography with ultraviolet and mass spectrometry detection for the analysis of microRNA biomarkers,” TALANTA, vol. 219, 2020.
@article{8732241, abstract = {{This study evaluates zwitterionic-hydrophilic interaction capillary liquid chromatography (capZIC-HILIC) and capillary electrophoresis (CE) with ultraviolet (UV) and mass spectrometry (MS) detection for the direct, label free and multiplex analysis of microribonucleic acids (miRNAs). CapZIC-HILIC-UV and CE-UV methods were first optimized, resulting in similar separations for a mixture of three miRNAs (hsa-iso-miR-16-5p, hsa-let-7g-5p, and hsa-miR-21-5p) but with reversal of elution/migration orders and small differences in repeatability, linearity, limit of detection (LOD) and separation efficiency. The established UV methods were transferred and validated in these terms with mass spectrometry (MS) detection, which allowed identifying the miRNAs and characterizing their post-transcriptional modifications. LOD by capZIC-HILIC-MS was 1 mu M of miRNA, around 5 times lower than by CE-MS due to the analyte dilution with the sheathflow CE-MS interface and to the slightly increased abundance of alkali metals adducts in the CE-MS mass spectra. In addition, the suction effect promoted by the nebulizer gas in CE-MS negatively affected the already compromised separations. In contrast, CE-MS showed superior repeatabilities with spiked serum samples, as well as reduced costs, extended capillary column durabilities and shorter conditioning times. The comparison of the different methods allows disclosing the current advantages and disadvantages of capZIC-HILIC and CE for the analysis of miRNA biomarkers.}}, articleno = {{121263}}, author = {{Vasconez, Jeanneth and Peró Gascón, Roger and Gimenez, Estela and Benavente, Fernando}}, issn = {{0039-9140}}, journal = {{TALANTA}}, keywords = {{Biomarker,Capillary electrophoresis,Capillary liquid chromatography,Mass spectrometry,miRNA,ZIC-HILIC,SERUM,OLIGONUCLEOTIDES,RETENTION,MIRNA,TIME}}, language = {{eng}}, pages = {{8}}, title = {{Comparison of capillary electrophoresis and zwitterionic-hydrophilic interaction capillary liquid chromatography with ultraviolet and mass spectrometry detection for the analysis of microRNA biomarkers}}, url = {{http://doi.org/10.1016/j.talanta.2020.121263}}, volume = {{219}}, year = {{2020}}, }
- Altmetric
- View in Altmetric
- Web of Science
- Times cited: