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Molting process revealed by the detailed expression profiles of RXR1/RXR2 and mining the associated genes in a spider mite, Panonychus citri

Gang Li, Xun‐Yan Liu, Guy Smagghe (UGent) , Jin‐Zhi Niu and Jin‐Jun Wang
(2022) INSECT SCIENCE. 29(2). p.430-442
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Abstract
Spider mites have one ecdysone receptor (EcR) and multiple retinoid X receptors (RXRs). However, the function of these RXRs in spider mite development is unknown. Here, we screened the expression dynamics of two PcRXR isoforms at 4 h intervals in the deutonymphal stage of Panonychus citri. The results showed that PcEcR had an expression pattern similar to that of PcRXR2. For PcRXR1, its expression remained at a certain high level, when there was a decrease of both PcEcR and PcRXR2. In situ hybridization showed that PcRXR2 was detected in the central nervous mass, while the ecdysteroid biosynthesis gene PcSpo was mainly expressed at the edge of the central nervous mass. RNAi-based silencing of PcRXR1 or PcRXR2 showed the same phenotype as in mites with that of silencing PcEcR. Furthermore, RNA-seq was used to mine the genes associated with the expression dynamics of PcRXR1 or PcRXR2, which revealed that the heterodimer of EcR-RXR2 in spider mites might be linked with the cell autophagy and tissue remodeling during apolysis, and RXR1 might be linked with new epicuticle and exocuticle secretion during ecdysis. Taken together, these results increase our understanding of the regulation mechanism of ecdysteroid signal pathway in spider mite development.
Keywords
ecdysone receptor (EcR), in situ hybridization, retinoid X receptors, RNA interference, RNA-seq, CUTICULAR PROTEIN GENES, RETINOID-X-RECEPTORS, TETRANYCHUS-URTICAE, ECDYSONE RECEPTORS, NERVOUS-SYSTEM, IDENTIFICATION, BIOSYNTHESIS, MORPHOLOGY, PATTERNS, INSECT

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MLA
Li, Gang, et al. “Molting Process Revealed by the Detailed Expression Profiles of RXR1/RXR2 and Mining the Associated Genes in a Spider Mite, Panonychus Citri.” INSECT SCIENCE, vol. 29, no. 2, 2022, pp. 430–42, doi:10.1111/1744-7917.12931.
APA
Li, G., Liu, X., Smagghe, G., Niu, J., & Wang, J. (2022). Molting process revealed by the detailed expression profiles of RXR1/RXR2 and mining the associated genes in a spider mite, Panonychus citri. INSECT SCIENCE, 29(2), 430–442. https://doi.org/10.1111/1744-7917.12931
Chicago author-date
Li, Gang, Xun‐Yan Liu, Guy Smagghe, Jin‐Zhi Niu, and Jin‐Jun Wang. 2022. “Molting Process Revealed by the Detailed Expression Profiles of RXR1/RXR2 and Mining the Associated Genes in a Spider Mite, Panonychus Citri.” INSECT SCIENCE 29 (2): 430–42. https://doi.org/10.1111/1744-7917.12931.
Chicago author-date (all authors)
Li, Gang, Xun‐Yan Liu, Guy Smagghe, Jin‐Zhi Niu, and Jin‐Jun Wang. 2022. “Molting Process Revealed by the Detailed Expression Profiles of RXR1/RXR2 and Mining the Associated Genes in a Spider Mite, Panonychus Citri.” INSECT SCIENCE 29 (2): 430–442. doi:10.1111/1744-7917.12931.
Vancouver
1.
Li G, Liu X, Smagghe G, Niu J, Wang J. Molting process revealed by the detailed expression profiles of RXR1/RXR2 and mining the associated genes in a spider mite, Panonychus citri. INSECT SCIENCE. 2022;29(2):430–42.
IEEE
[1]
G. Li, X. Liu, G. Smagghe, J. Niu, and J. Wang, “Molting process revealed by the detailed expression profiles of RXR1/RXR2 and mining the associated genes in a spider mite, Panonychus citri,” INSECT SCIENCE, vol. 29, no. 2, pp. 430–442, 2022.
@article{8741554,
  abstract     = {{Spider mites have one ecdysone receptor (EcR) and multiple retinoid X receptors (RXRs). However, the function of these RXRs in spider mite development is unknown. Here, we screened the expression dynamics of two PcRXR isoforms at 4 h intervals in the deutonymphal stage of Panonychus citri. The results showed that PcEcR had an expression pattern similar to that of PcRXR2. For PcRXR1, its expression remained at a certain high level, when there was a decrease of both PcEcR and PcRXR2. In situ hybridization showed that PcRXR2 was detected in the central nervous mass, while the ecdysteroid biosynthesis gene PcSpo was mainly expressed at the edge of the central nervous mass. RNAi-based silencing of PcRXR1 or PcRXR2 showed the same phenotype as in mites with that of silencing PcEcR. Furthermore, RNA-seq was used to mine the genes associated with the expression dynamics of PcRXR1 or PcRXR2, which revealed that the heterodimer of EcR-RXR2 in spider mites might be linked with the cell autophagy and tissue remodeling during apolysis, and RXR1 might be linked with new epicuticle and exocuticle secretion during ecdysis. Taken together, these results increase our understanding of the regulation mechanism of ecdysteroid signal pathway in spider mite development.}},
  author       = {{Li, Gang and Liu, Xun‐Yan and Smagghe, Guy and Niu, Jin‐Zhi and Wang, Jin‐Jun}},
  issn         = {{1672-9609}},
  journal      = {{INSECT SCIENCE}},
  keywords     = {{ecdysone receptor (EcR),in situ hybridization,retinoid X receptors,RNA interference,RNA-seq,CUTICULAR PROTEIN GENES,RETINOID-X-RECEPTORS,TETRANYCHUS-URTICAE,ECDYSONE RECEPTORS,NERVOUS-SYSTEM,IDENTIFICATION,BIOSYNTHESIS,MORPHOLOGY,PATTERNS,INSECT}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{430--442}},
  title        = {{Molting process revealed by the detailed expression profiles of RXR1/RXR2 and mining the associated genes in a spider mite, Panonychus citri}},
  url          = {{http://doi.org/10.1111/1744-7917.12931}},
  volume       = {{29}},
  year         = {{2022}},
}
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