Analysis of ROS-triggered changes in the transcriptome
(2022)
Reactive oxygen species in plants : methods and protocols.
In Methods in Molecular Biology
2526.
p.277-288
- Author
- Patrick Willems (UGent)
- Organization
- Abstract
- RNA sequencing is routinely used for determining transcriptome-wide expression changes during various conditions, including oxidative stress conditions. In this chapter, a basic workflow to determine differentially expressed genes between two conditions of interest is provided. After providing brief guidelines for experimental design, we provide step-by-step instructions for genome alignment of reads and differential expression analysis.
- Keywords
- Differential gene expression, Genome alignment, Next-generation sequencing, Reactive oxygen species, Transcriptomics, edgeR, mRNA-sequencing
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8756677
- MLA
- Willems, Patrick. “Analysis of ROS-Triggered Changes in the Transcriptome.” Reactive Oxygen Species in Plants : Methods and Protocols, edited by Amna M’Hamdi, vol. 2526, Humana, 2022, pp. 277–88, doi:10.1007/978-1-0716-2469-2_20.
- APA
- Willems, P. (2022). Analysis of ROS-triggered changes in the transcriptome. In A. M’Hamdi (Ed.), Reactive oxygen species in plants : methods and protocols (Vol. 2526, pp. 277–288). https://doi.org/10.1007/978-1-0716-2469-2_20
- Chicago author-date
- Willems, Patrick. 2022. “Analysis of ROS-Triggered Changes in the Transcriptome.” In Reactive Oxygen Species in Plants : Methods and Protocols, edited by Amna M’Hamdi, 2526:277–88. New York: Humana. https://doi.org/10.1007/978-1-0716-2469-2_20.
- Chicago author-date (all authors)
- Willems, Patrick. 2022. “Analysis of ROS-Triggered Changes in the Transcriptome.” In Reactive Oxygen Species in Plants : Methods and Protocols, ed by. Amna M’Hamdi, 2526:277–288. New York: Humana. doi:10.1007/978-1-0716-2469-2_20.
- Vancouver
- 1.Willems P. Analysis of ROS-triggered changes in the transcriptome. In: M’Hamdi A, editor. Reactive oxygen species in plants : methods and protocols. New York: Humana; 2022. p. 277–88.
- IEEE
- [1]P. Willems, “Analysis of ROS-triggered changes in the transcriptome,” in Reactive oxygen species in plants : methods and protocols, vol. 2526, A. M’Hamdi, Ed. New York: Humana, 2022, pp. 277–288.
@incollection{8756677,
abstract = {{RNA sequencing is routinely used for determining transcriptome-wide expression changes during various conditions, including oxidative stress conditions. In this chapter, a basic workflow to determine differentially expressed genes between two conditions of interest is provided. After providing brief guidelines for experimental design, we provide step-by-step instructions for genome alignment of reads and differential expression analysis.}},
author = {{Willems, Patrick}},
booktitle = {{Reactive oxygen species in plants : methods and protocols}},
editor = {{M'Hamdi, Amna}},
isbn = {{9781071624685}},
issn = {{1064-3745}},
keywords = {{Differential gene expression,Genome alignment,Next-generation sequencing,Reactive oxygen species,Transcriptomics,edgeR,mRNA-sequencing}},
language = {{eng}},
pages = {{277--288}},
publisher = {{Humana}},
series = {{Methods in Molecular Biology}},
title = {{Analysis of ROS-triggered changes in the transcriptome}},
url = {{http://doi.org/10.1007/978-1-0716-2469-2_20}},
volume = {{2526}},
year = {{2022}},
}
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