Functional characterization of glycine effects on oligodendroglial cell lines

Abstract

In this study the effect of glycine on 3 oligodendroglial cell lines was investigated. Patch-clamp results suggest that mainly Na+-dependent-like transporters are responsible for the glycine-induced inward current in the used cell lines. In the MO3.13 and OLN-93 cells there is also a small contribution of GlyT2s to the currents. When looking at the effect of glycine on cell proliferation, there is only an effect on the MO3.13 cells. Since the glycine-induced current in the MO3.13 cells is approximately six times larger than those induced in the HOG and OLN-93 cells, it is likely that the currents in the latter two are too small to trigger any downstream events. These results suggest that glycine can influence the proliferation of some, but not all, oligodendroglial cell lines. Verification of these results with primary cell cultures will give more information concerning the use of these cell lines as model for oligodendendrocyte progenitors.