The aim of this work was the preparation and characterization of cationic Solid Lipid Nanoparticles (cSLN) containing erythromycin (ERY), as a potential tool to improve the penetration and accumulation of the antibiotic into bacteria cells. Using a solvent injection method, homogeneous populations of lipid nanoparticles with an average size between 250 and 400 nm and with a polydispersity index lower than 0.3 were obtained; positive zeta potential values were granted by addition of the cationic lipid quaternary ammonium salt didecyldimethylammonium bromide (DDAB). Stability studies showed that the formulations were almost stable for up to one year, depending on the storage temperature. In vitro microbiological studies confirmed the antimicrobial activity of the SLN, providing Minimal Inhibitory Concentration (MIC) values for drug loaded cSLN comparable to unencapsulated ERY. In particular, the presence of increasing concentrations of DDAB in the SLN matrix contributed to the antimicrobial activity of the nanocarriers, although elucidation of the mechanism underlying this interaction would deserve supplementary investigation.
Formulation and characterization of erythromycin–loaded Solid Lipid Nanoparticles
Pignatello R.
;Fuochi V.;Petronio Petronio G.;Furneri P. M.
2017-01-01
Abstract
The aim of this work was the preparation and characterization of cationic Solid Lipid Nanoparticles (cSLN) containing erythromycin (ERY), as a potential tool to improve the penetration and accumulation of the antibiotic into bacteria cells. Using a solvent injection method, homogeneous populations of lipid nanoparticles with an average size between 250 and 400 nm and with a polydispersity index lower than 0.3 were obtained; positive zeta potential values were granted by addition of the cationic lipid quaternary ammonium salt didecyldimethylammonium bromide (DDAB). Stability studies showed that the formulations were almost stable for up to one year, depending on the storage temperature. In vitro microbiological studies confirmed the antimicrobial activity of the SLN, providing Minimal Inhibitory Concentration (MIC) values for drug loaded cSLN comparable to unencapsulated ERY. In particular, the presence of increasing concentrations of DDAB in the SLN matrix contributed to the antimicrobial activity of the nanocarriers, although elucidation of the mechanism underlying this interaction would deserve supplementary investigation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
