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Abstract:

Domestic dogs and cats are major domestic reservoir hosts of Trypanosoma cruzi and a risk factor for parasite transmission. In this study we assessed the relative performance of a polymerase chain reaction assay targeted to minicircle DNA (kDNA-PCR) in reference to conventional serological tests, a rapid dipstick test and xenodiagnosis to detect T. cruzi infection in dogs and cats from an endemic rural area in northeastern Argentina. A total of 43 dogs and 13 cats seropositive for T. cruzi by an immunosorbent assay (ELISA) and an indirect hemagglutination assay (IHA), which had been examined by xenodiagnosis, were also tested by kDNA-PCR. kDNA-PCR was nearly as sensitive as xenodiagnosis for detecting T. cruzi-infectious dogs and cats. kDNA-PCR was slightly more sensitive than xenodiagnosis in seropositive dogs (91% versus 86%, respectively) and cats (77% against 54%, respectively), but failed to detect all of the seropositive individuals. ELISA and IHA detected all xenodiagnosis-positive dogs and both outcomes largely agreed (kappa coefficient, κ= 0.92), whereas both assays failed to detect all of the xenodiagnosis-positive cats and their agreement was moderate (κ= 0.68). In dogs, the sensitivity of the dipstick test was 95% and agreed closely with the outcome of conventional serological tests (κ= 0.82). The high sensitivity of kDNA-PCR to detect T. cruzi infections in naturally infected dogs and cats supports its application as a diagnostic tool complementary to serology and may replace the use of xenodiagnosis or hemoculture. © 2013 Elsevier B.V.

Registro:

Documento: Artículo
Título:Detection of Trypanosoma cruzi infection in naturally infected dogs and cats using serological, parasitological and molecular methods
Autor:Enriquez, G.F.; Cardinal, M.V.; Orozco, M.M.; Schijman, A.G.; Gürtler, R.E.
Filiación:Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, Buenos Aires, Argentina
Laboratorio de Biología Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genetica y Biologia Molecular (INGEBI-CONICET), Vuelta de Obligado 2490, Buenos Aires, Argentina
Palabras clave:Chagas disease; Polymerase chain reaction; Reservoir; Serodiagnosis; Trypanosoma cruzi; Xenodiagnosis; minicircle DNA; canid; detection method; disease incidence; DNA; felid; immunoassay; infectivity; parasite transmission; polymerase chain reaction; risk factor; rural area; trypanosomiasis; animal experiment; Argentina; article; cat disease; Chagas disease; controlled study; cross-sectional study; diagnostic test accuracy study; dog disease; enzyme linked immunosorbent assay; hemagglutination test; indirect hemagglutination assay; intermethod comparison; molecular diagnosis; nonhuman; parasite serodiagnosis; polymerase chain reaction; rapid dipstick test; rural area; sensitivity analysis; Trypanosoma cruzi; xenodiagnosis; Animals; Argentina; Cat Diseases; Cats; Chagas Disease; Diagnostic Tests, Routine; Dog Diseases; Dogs; Molecular Diagnostic Techniques; Parasitology; Sensitivity and Specificity; Serologic Tests; Trypanosoma cruzi; Veterinary Medicine; Argentina; Canis familiaris; Trypanosoma cruzi
Año:2013
Volumen:126
Número:3
Página de inicio:211
Página de fin:217
DOI: http://dx.doi.org/10.1016/j.actatropica.2013.03.001
Título revista:Acta Tropica
Título revista abreviado:Acta Trop.
ISSN:0001706X
CODEN:ACTRA
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0001706X_v126_n3_p211_Enriquez

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Citas:

---------- APA ----------
Enriquez, G.F., Cardinal, M.V., Orozco, M.M., Schijman, A.G. & Gürtler, R.E. (2013) . Detection of Trypanosoma cruzi infection in naturally infected dogs and cats using serological, parasitological and molecular methods. Acta Tropica, 126(3), 211-217.
http://dx.doi.org/10.1016/j.actatropica.2013.03.001
---------- CHICAGO ----------
Enriquez, G.F., Cardinal, M.V., Orozco, M.M., Schijman, A.G., Gürtler, R.E. "Detection of Trypanosoma cruzi infection in naturally infected dogs and cats using serological, parasitological and molecular methods" . Acta Tropica 126, no. 3 (2013) : 211-217.
http://dx.doi.org/10.1016/j.actatropica.2013.03.001
---------- MLA ----------
Enriquez, G.F., Cardinal, M.V., Orozco, M.M., Schijman, A.G., Gürtler, R.E. "Detection of Trypanosoma cruzi infection in naturally infected dogs and cats using serological, parasitological and molecular methods" . Acta Tropica, vol. 126, no. 3, 2013, pp. 211-217.
http://dx.doi.org/10.1016/j.actatropica.2013.03.001
---------- VANCOUVER ----------
Enriquez, G.F., Cardinal, M.V., Orozco, M.M., Schijman, A.G., Gürtler, R.E. Detection of Trypanosoma cruzi infection in naturally infected dogs and cats using serological, parasitological and molecular methods. Acta Trop. 2013;126(3):211-217.
http://dx.doi.org/10.1016/j.actatropica.2013.03.001