Navegar

Colección

Datos Estadísticas

Artículo

La versión final de este artículo es de uso interno. El editor solo permite incluir en el repositorio el artículo en su versión post-print. Por favor, si usted la posee enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Supramolecular organized multilayers composed of glucose oxidase (GOx) and osmium-derivatized poly(allylamine) redox polymer have been self-assembled electrostatically from Os-polyelectrolyte solutions of variable pH (5.5-8.8) leading to a decrease of the linear charge density in the PAH-Os with increasing pH. The layer-by-layer enzyme multilayers were studied by ellipsometry, quartz crystal microbalance, AFM, cyclic voltammetry, and electrocatalytic oxidation of β-D-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0-7.5 where a change in the film growth mechanism is observed. © 2006 American Chemical Society.

Registro:

Documento: Artículo
Título:Structure and thickness dependence of "molecular wiring" in nanostructured enzyme multilayers
Autor:Flexer, V.; Forzani, E.S.; Calvo, E.J.; Ludueña, S.J.; Pietrasanta, L.I.
Filiación:Departamento de Química Inorgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Centro de Microscopias Avanzadas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:AFM; Electrocatalytic oxidation; Glucose oxidase (GOx); Quartz crystal microbalance; Adsorption; Cyclic voltammetry; Ellipsometry; Nanostructured materials; Oxidation; Polymers; Quartz applications; Structural analysis; Enzyme kinetics; allylamine; bipyridine derivative; glucose; glucose oxidase; nanoparticle; osmium derivative; polyallylamine; polyelectrolyte; silicon dioxide; unclassified drug; adsorption; article; atomic force microscopy; catalysis; cyclic potentiometry; electrode; ellipsometry; enzyme electrode; enzyme structure; film; oxidation; oxidation reduction reaction; pH; supramolecular chemistry; thickness; Electrochemistry; Glucose; Glucose Oxidase; Microscopy, Atomic Force; Nanostructures; Organometallic Compounds; Osmium; Oxidation-Reduction; Polyamines
Año:2006
Volumen:78
Número:2
Página de inicio:399
Página de fin:407
DOI: http://dx.doi.org/10.1021/ac050973n
Título revista:Analytical Chemistry
Título revista abreviado:Anal. Chem.
ISSN:00032700
CODEN:ANCHA
CAS:allylamine, 107-11-9; glucose, 50-99-7, 84778-64-3; glucose oxidase, 9001-37-0; silicon dioxide, 10279-57-9, 14464-46-1, 14808-60-7, 15468-32-3, 60676-86-0, 7631-86-9; Glucose, 50-99-7; Glucose Oxidase, 1.1.3.4; Organometallic Compounds; Osmium, 7440-04-2; Polyamines; polyallylamine, 30551-89-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00032700_v78_n2_p399_Flexer

Referencias:

  • Schuhmann, W., (2002) Rev. Mol. Biotechnol., 82, pp. 425-441
  • Heller, A., (1990) Acc. Chem, Res., 23, pp. 128-134
  • Hodak, J., Etchenique, R., Calvo, E.J., Singhal, K., Bartlett, P.N., (1997) Langmuir, 13, pp. 2708-2716
  • Anicet, N., Bourdillon, C., Moiroux, J., Saveant, J.-M., (1999) Langmuir, 15, pp. 6527-6533
  • Willner, I., Lapidot, N., Riklin, A., Kasher, R., Zahavy, E., Eugenii, K., (1994) J. Am. Chem. Soc., 116, pp. 1428-1441
  • Sun, S., Ho-Si, P.H., Harrison, D.J., (1991) Langmuir, 7, pp. 727-737
  • Guiomar, A.J., Guthrie, J.T., Evans, S.D., (1999) Langmuir, 15, pp. 1198-1207
  • Onda, M., Lvov, Y., Ariga, K., Kunitake, J., (1996) J. Ferment. Bioeng., 82, p. 502
  • Willner, I., Katz, E., Willner, B., (1997) Electroanalysis, 9, pp. 965-977
  • Calvo, E.J., Etchenique, R., Pietrasanta, L., Wolosiuk, A., Danilowicz, C., (2001) Anal. Chem., 73, pp. 1161-1168
  • Blonder, R., Katz, E., Cohen, Y., Itzhak, N., Riklin, A., Willner, I., (1996) Anal. Chem., 68, pp. 3151-3157
  • Bourdillon, C., Demaille, C., Moiroux, J., Saveant, J.-M., (1996) Acc. Chem. Res., 29, pp. 529-535
  • Anicet, N., Bourdillon, C., Moiroux, J., Saveant, J.-M., (1998) J. Phys. Chem. B, 102, pp. 9844-9849
  • Rusling, J.F., (1998) Acc. Chem. Res., 31, pp. 363-369
  • Franchina, J.G.L., Lackowski, W.M., Dermody, D.L., Crooks, R.M., Bergbreiter, D.E., Sirkar, K., Russell, R.J., Pishko, M.V., (1999) Anal. Chem., 71, pp. 3133-3139
  • Yoon, H.C., Kim, H.-S., (2000) Anal. Chem., 72, pp. 922-926
  • Willner, I., Heleg-Shabtai, V., Blonder, R., Katz, E., Tao, G., Buckmann, A., Heller, A., (1996) J. Am. Chem. Soc., 118, pp. 10321-10322
  • Forzani, E.S., Otero, M., Perez, M.A., Teijelo, M.L., Calvo, E.J., (2002) Langmuir, 18, pp. 4020-4029
  • Calvo, E.J., Forzani, E., Otero, M., (2002) J. Electroanal Chem., 538, pp. 231-241
  • Forzani, E.S., Perez, M.A., Lopez Teijelo, M., Calvo, E.J., (2002) Langmuir, 18, pp. 9867-9873
  • Bonazzola, C., Calvo, E.J., Nart, F.C., (2003) Langmuir, 19, pp. 5279-5286
  • Tognalli, N., Fainstein, A., Bonazzola, C., Calvo, E., (2004) J. Chem. Phys., 120, pp. 1905-1911
  • Calvo, E.J., Danilowicz, C., Wolosiuk, A., (2002) J. Am. Chem. Soc., 124, pp. 2452-2453
  • Calvo, E.J., Wolosiuk, A., (2004) ChemPhysChem, 5, pp. 235-239
  • Calvo, E.J., Danilowicz, C.B., Wolosiuk, A., (2005) Phys. Chem. Chem. Phys., 7, pp. 1-7
  • Decher, G., (2003) Multilayer Thin Films, , Decher, G., Schlenoff, J. B., Eds.; Weinheim: Wiley-VCH, Chapter 1
  • Shiratori, S.S., Rubner, M.F., (2000) Macromolecules, 33, pp. 4213-4219
  • Danilowicz, C., Corton, E., Battaglini, F., (1998) J. Electroanal. Chem., 445, pp. 89-94
  • Angerstein-Kozlowka, H., Conway, B.E., Barnett, B., Mozota, J., (1979) J. Electroanal. Chem., 100, pp. 417-446
  • Sawyer, D.T., Roberts, J.L., (1974) J. Experimental Electrochemistry for Chemists, , Wiley: New York
  • Calvo, E.J., Danilowicz, C., Etchenique, R.J., (1995) Chem. Soc., Faraday Trans., 91, pp. 4083-4091
  • Yoshikawa, Y., Matsuoka, H., Ise, N., (1986) Br. Polym. J., 18, pp. 242-246
  • Petrov, A.I., Antipov, A.A., Sukhorukov, G.B., (2003) Macromolecules, 36, pp. 10079-10086
  • Suh, J., Paik, H.J., Hwang, B.K., (1994) Bioorg. Chem., 22, pp. 318-327
  • Choi, J., Rubner, M.F., (2005) Macromolecules, 38, pp. 116-124
  • Ochiai, H., Anabuki, Y., Kojima, O., Tominaga, K., Murakami, I., (1990) J. Polym, Sci., Part B: Polym. Phys., 28, pp. 233-240
  • Fang, M., Kim, C.H., Saupe, G.B., Kim, H.-N., Waraksa, C.C., Miwa, T., Fujishima, A., Mallouk, T.E., (1999) Chem. Mater., 11, pp. 1526-1532
  • Voet, J.G., Coe, J., Epstein, J., Matossian, V., Shipley, T., (1981) Biochemistry, 20, pp. 7182-7185
  • Calvo, E.J., Wolosiuk, A., (2005) ChemPhysChem, 6, pp. 43-47
  • Calvo, E.J., Battaglini, F., Danilowicz, C., Wolosiuk, A., Otero, M., (2000) Faraday Discuss., 116, pp. 47-65. , discussion 67-75
  • Calvo, E.J., Forzani, E.S., Otero, M., (2002) Anal. Chem., 74, pp. 3281-3289
  • Sauerbrey, G.Z., (1959) Z. Phys., 155, pp. 206-222
  • Caruso, F., Furlong, D.N., Ariga, K., Ichinose, I., Kunitake, T., (1998) Langmuir, 14, pp. 4559-4565
  • Lobo, R.F.M., Pereira-da-Silva, M.A., Paposo, M., Faria, R.M., Oliveira Jr., O.N., (2003) Nanotechnology, 14, pp. 101-108
  • Limoges, B., Moiroux, J., Savéant, J.-M., (2002) J. Electroanal. Chem., 521, pp. 1-7
  • Limoges, B., Moiroux, J., Savéant, J.-M., (2002) J. Electroanal. Chem., 521, pp. 8-15
  • Bartlett, P.N., Pratt, K.F.E., (1995) J. Electroanal. Chem., 397, pp. 61-78
  • Weibel, M., Bright, H.J., (1971) Biochem. J., 124, p. 801
  • Harris, J.J., Stair, J.L., Bruening, M.L., (2000) Chem. Mater., 12, pp. 1941-1946
  • Dai, J., Jensen, A.W., Mohanty, D.K., Erndt, J., Bruening, M.L., (2001) Langmuir, 17, pp. 931-937
  • Liu, X., Bruening, M.L., (2004) Chem. Mater., 16, pp. 351-357
  • Farhat, T.R., Schlenoff, J.B., (2001) Langmuir, 17, pp. 1184-1192
  • Krasemann, L., Tieke, B., (2000) Langmuir, 16, pp. 287-290
  • Wilson, R., Turner, A.P.F., (1992) Biosens. Bioelectron., 7, pp. 165-185
  • Bartlett, P.N., Pratt, K.F.E., (1995) J. Electroanal. Chem., 397, pp. 53-60
  • Bourdillon, C., Demaille, C., Moiroux, J., Saveant, J.M., (1993) J. Am. Chem. Soc., 115, pp. 2-10
  • Yokoyama, K., Kayanuma, Y., (1998) Anal. Chem., 70, pp. 3368-3376
  • Bright, H.J., Appleby, M., (1969) J. Biol. Chem., 244, pp. 3625-3634
  • Gibson, Q.H., Swoboda, B.E., Massey, V., (1964) J. Biol. Chem., 239, pp. 3927-3934
  • Flexer, V.; Calvo, E. J. Unpublished results, 2005. More recent measurements yielded the value 360 s-1

Citas:

---------- APA ----------
Flexer, V., Forzani, E.S., Calvo, E.J., Ludueña, S.J. & Pietrasanta, L.I. (2006) . Structure and thickness dependence of "molecular wiring" in nanostructured enzyme multilayers. Analytical Chemistry, 78(2), 399-407.
http://dx.doi.org/10.1021/ac050973n
---------- CHICAGO ----------
Flexer, V., Forzani, E.S., Calvo, E.J., Ludueña, S.J., Pietrasanta, L.I. "Structure and thickness dependence of "molecular wiring" in nanostructured enzyme multilayers" . Analytical Chemistry 78, no. 2 (2006) : 399-407.
http://dx.doi.org/10.1021/ac050973n
---------- MLA ----------
Flexer, V., Forzani, E.S., Calvo, E.J., Ludueña, S.J., Pietrasanta, L.I. "Structure and thickness dependence of "molecular wiring" in nanostructured enzyme multilayers" . Analytical Chemistry, vol. 78, no. 2, 2006, pp. 399-407.
http://dx.doi.org/10.1021/ac050973n
---------- VANCOUVER ----------
Flexer, V., Forzani, E.S., Calvo, E.J., Ludueña, S.J., Pietrasanta, L.I. Structure and thickness dependence of "molecular wiring" in nanostructured enzyme multilayers. Anal. Chem. 2006;78(2):399-407.
http://dx.doi.org/10.1021/ac050973n